Method for rapidly propagating sprouts of chirita lutea by using chirita lutea leaves

A technology of yellow-flowered cow ears and leaves, applied in the biological field, to achieve the effects of easy acquisition, short seedling time and low production cost

Inactive Publication Date: 2015-06-03
GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the technology of rapid propagation with the leaves of Cow's ears as explants

Method used

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  • Method for rapidly propagating sprouts of chirita lutea by using chirita lutea leaves

Examples

Experimental program
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Effect test

Embodiment 1

[0020] A method for rapidly propagating its seedlings by utilizing the leaves of Cow's ears of yellow flowers, comprising the steps of:

[0021] (1) Treatment of explants: Take the leaves of Cow's ears as explants, wash off the dust on the surface with water, put them into 50% carbendazim WP 1g / L+ascorbic acid 0.8g / L+penicillin 100 IU / L + streptomycin 100μg / L, soak in the mixed disinfectant solution for 15-20min, wash with detergent for 1min, and rinse with running water for 15-20min; transfer to ultra-clean bench, soak in 75% alcohol for 6-10s, Rinse with sterile water 2 to 3 times, then soak and disinfect with 0.1% mercuric acid solution for 7 to 10 minutes, rinse with sterile water for 3 to 4 times, and finally dry the surface moisture of explants with sterile filter paper;

[0022] (2) Primary culture of explants: cut the treated explants into small pieces of 1.5 cm×1.0 cm with a scalpel after being burned at high temperature and cooled, aired in the ultra-clean workbenc...

Embodiment 2

[0026] A method for quickly propagating its seedlings by utilizing the leaves of Cow's ears of yellow flowers, comprising the method steps of embodiment 1, the difference is: the explants of the primary culture are inoculated with MS+TDZ0.1 mg / L+6-BA0.5mg / L+NAA0.5mg / L+sucrose 30g / L+agar 6.5g / L in medium. On the 13th day, a large number of small buds germinated on the edge of the leaves and on the surface of the explants, and the buds were scattered on the explants. The buds were strong and light green, and the induction rate was 66.7%. After culturing for 50 days, the proliferation rate was counted, and the number of seedlings induced by a single leaf was 35-80.

Embodiment 3

[0028] A method for rapidly propagating its seedlings by utilizing the leaves of Cow's ears of yellow flowers, comprising the method steps of embodiment 1, the difference is: the explants of the primary culture are inoculated in MS+6-BA0.1mg / L+NAA0.05mg / L + sucrose 30g / L + agar 6.5g / L medium. On the 19th day, small buds began to be induced at the edge of the incision, and the induction rate was 100%. After culturing for 50 days, the proliferation rate was counted, and the number of seedlings induced by a single leaf was 55-75.

[0029] The following table shows the effect of different mediums on the explant induction rate, bud germination time, single explant induced bud number and transplanting survival rate of Cattle ear tissue culture rapid propagation in Examples 1 to 3 during primary culture. influences.

[0030]

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Abstract

The invention discloses a method for rapidly propagating sprouts of chirita lutea by using chirita lutea leaves. Primary culture on explants, subculture and hardening and transplanting are carried out, and seedlings are directly obtained by adopting a two-step method including the primary culture and the subculture, a large number of strong seedlings can be obtained through propagation within about 90 days without a rooting phase, and the subculture is carried out without adding a hormone, and thus the production link is reduced, the production cost is lowered, and the effective propagation of the chirita lutea is realized. The method brings the convenience to collect materials without influencing the quantity of wild populations of the chirita lutea; the method is simple to operate, low in production cost, stable and efficient, and capable of greatly shortening the seedling growing period, and provides a technical support for protection and development and utilization of the chirita lutea.

Description

technical field [0001] The invention relates to a method for in vitro rapid propagation of the rare and endangered plant Cow's ear. Specifically, the leaves of the Cow's ear are used as explants for tissue culture to obtain test-tube seedlings, and belong to the field of biotechnology. Background technique [0002] Yellow cow ears ( Chirita lutea ) is a plant of Gesneriaceae (Gesneriaceae) endemic to Guangxi, which has the effects of clearing the lung and relieving cough, tonifying deficiency and stopping bleeding. It can cure tuberculosis, cough and asthma, hematemesis, red metrorrhagia and leucorrhea. At present, it is only found to be distributed on limestone mountains in some areas of Hezhou, Guangxi. Due to the random excavation of the people in the production area, the continuous deterioration of the ecological environment in the limestone area and other external factors, as well as the low natural reproductive capacity, the number of wild populations of yellow-flow...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 梁惠凌吴群英孔德鑫葛玉珍林军孙志会龙晶刘燕飞
Owner GUANGXI INST OF BOTANY THE CHINESE ACAD OF SCI
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