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Method for aseptic sowing and seedling raising of phalaenopsis and rhynchostylis retusa distant hybridization seeds

A technology of distant hybridization and aseptic sowing, which is applied in sowing, horticultural methods, botanical equipment and methods, etc., can solve problems that have not been seen, and achieve improved survival rate, increased seedling rate, and good seedling quality Effect

Active Publication Date: 2020-10-13
ENVIRONMENTAL HORTICULTURE RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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  • Application Information

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Problems solved by technology

At present, there are no research reports on aseptic sowing and seedling cultivation of Phalaenopsis and Orchid orchids in distant hybrids at home and abroad

Method used

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Embodiment Construction

[0025] In view of this, a method for aseptic sowing and seedling cultivation of Phalaenopsis and Phalaenopsis distant hybrid seeds provided in the specific embodiment of the present invention specifically includes the following steps:

[0026] (1) Artificial pollination: take Phalaenopsis as the female parent, orchid orchid as the male parent to hybridize, select fresh and energetic flowers, remove the pollen pieces on the lip and symphyllum of the female parent with sterilized tweezers, and take The fresh pollen mass of the male parent is placed in the stamen cavity of the female parent; 100-120 days after pollination, the uncracked mature fruit pods are harvested for aseptic sowing;

[0027] (2) Fruit pod pretreatment and seed sowing: After the fruit pods are washed with running water, they are scrubbed with 75% alcohol on the ultra-clean workbench, and then cleaned with 0.1% HgCl 2 Sterilize the solution for 10-12 minutes, rinse with sterile water for 3 to 5 times, cut off ...

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Abstract

The invention provides a method for aseptic sowing and seedling raising of phalaenopsis and rhynchostylis retusa distant hybridization seeds. Phalaenopsis serves as a female parent, rhynchostylis retusa serves as a male parent, hybrid pods are obtained through artificial pollination, and distant hybrid progeny populations are obtained through the steps of pod pretreatment, aseptic sowing of seeds,seed germination, protocorm proliferation and differentiation, strong seedling rooting, acclimatization and transplantation and other steps. The method has the advantages of high seed germination rate, large seedling-forming number, short seedling-forming time, good seedling quality and the like, and the problems that distant hybridization seeds are not easy to successfully germinate due to affinity and are extremely difficult to germinate under natural conditions, and seedlings are not easy to grow are solved. Therefore, a large number of seedlings can be obtained in a short period through an aseptic sowing and artificial propagation method, and a foundation is laid for breeding of phalaenopsis distant hybridization excellent varieties.

Description

technical field [0001] The invention belongs to the technical field of tissue culture rapid propagation, and in particular relates to a method for aseptic sowing and seedling raising of distant hybrid seeds of Phalaenopsis orchid orchid. Background technique [0002] Phalaenopsis is known as the "Queen of Orchids" because of its unique flower shape, gorgeous flower color, rich color, neat inflorescence and long flowering period. Phalaenopsis is currently the most sold orchid variety in the world. Among the orchid sales in the United States, Phalaenopsis accounts for 75% of all orchids. Consumption has exceeded 280 million plants. In recent years, the hybrid breeding of Phalaenopsis has made great breeding achievements and made significant contributions to the development of the industry, but the genetic basis of Phalaenopsis is still relatively narrow. Therefore, it is of great theoretical and practical significance to introduce excellent genes from intergeneric wild germp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H4/00A01G31/00
CPCA01H1/02A01H4/008A01H4/001A01G31/00A01G22/63A01H6/62A01H5/02A01H1/021A01G9/0293A01C7/00A01D46/00A01G9/246
Inventor 陈和明吕复兵肖文芳李佐
Owner ENVIRONMENTAL HORTICULTURE RES INST OF GUANGDONG ACADEMY OF AGRI SCI
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