Commercial production method of Dendrobium drumstick seedlings

Abstract

The invention discloses a commercial production method of Dendrobium pachyrhiza seedlings, which belongs to the field of plant biotechnology. The present invention uses the fruit pods of Dendrobium chrysalis as explants, adopts the improved Ma basic medium, adds peeled potato mash and banana mash at each stage of cultivation, and establishes the aseptic and rapid propagation technology system of Dendrobium chrysalis, including improved varieties Related technologies such as breeding, medium preparation, bottle seedling cultivation, and pseudo-planting cultivation can effectively solve the problem of the source of excellent seedlings of Dendrobium drumsticks and the purity of seedlings. Compared with conventional seedling tissue culture and rapid propagation, the seed germination rate Increase to more than 98%, reduce production cost by 20%, shorten the growth cycle by 60 days, reduce the pollution rate by 5%, increase the survival rate of hardening and transplanting to more than 95%, and cultivate fake plants for 60-90 days, which can be sold or transplanted Planting, compared with the planting of ramet propagation seedlings, the present invention cultivates Dendrobium pachyrhiza seedlings planting and increases 5~7 strains of freshly drawn seedlings of each clump.

Description

technical field [0001] The invention relates to a method for commercially producing seedlings of the edible and medicinal plant Dendrobium pachyrhiza, belonging to the field of plant biotechnology. Background technique [0002] Dendrobium chrysotoxum (Dendrobium chrysotoxum) is a perennial epiphytic herb of the genus Dendrobium orchidaceae. The ornamental value is very high. It can be used as cut flowers and potted plants. The flowers can also be used to develop a series of products such as Dendrobium chrysalis tea, wine, flower cakes, etc. Dendrobium chrysalis is rich in flower resources. It is reported that it contains 22 compounds, which have the functions of improving eyesight and calming the nerves. It has the effects of nourishing blood, anti-cancer and relieving depression, and lowering the three high levels; its stems can be used medicinally, and have the functions of clearing away heat and detoxification, and enhancing immunity. Due to the large differences in the f...

AI Technical Summary

Problems solved by technology

[0003] In recent years, there have been some reports on the tissue culture of Dendrobium chrysalis, and certain tissue culture seedlings have been obtained through tissue culture experiments. However, in the large-scale production technology of Dendrobium, the roots of the cultivated seedlings are thin and few, and the germination rate of the embryos is 75% low. %, the pollution rate is up to 10%, the seedling propagation cycle is long (more than 8 months), and the seedling production cost is up to 1.2 yuan / clump, therefore, carry out the tissue culture breeding research of the sex line of Dendrobium chrysalis, improve the species of Dendrobium chrysalis Seedling commercial production technology also has the following problems:

[0004] 1. Due to the large differences in the traits of the germplasm resources of Dendrobium chrysalis, and relatively stable heredity in terms of yield, the growth of stems, leaves, and flowers is significantly different. Dendrobium chrysalis bears natural fruit. In the absence of light and water and fertilizer, the development of fruit pods is small But not good, the purity of the variety is low, resulting in low germination rate, slow multiplication rate, and poor quality of seedlings

[0005] 2. At present, the seedlings of Dendrobium chrysalis are mainly propagated by division, which leads to a large waste of raw materials and a low germination rate, which is difficult to meet the market demand; while the existing tissue culture technology of Dendrobium chrysalis uses the pods of natural results as explants However, problems such as low germination rate of seed embryos, slow seedling production speed, serious seedling yellowing, and low multiplication multiples lead to slow seedling production speed, poor quality of rooted seedlings, low survival rate of hardening and transplanting, and slow growth. impact on the scale and speed of commercial production of Dendrobium chrysanthemum seedlings