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New Use of Dendrobium Polypeptide

A Dendrobium and medicine technology, which is applied in the direction of medical preparations containing active ingredients, toiletry preparations, peptide/protein components, etc., can solve the problem that the drug effect of protein is not known, so as to improve the additional utilization rate and improve the non-specific The effect of heterosexual immune function and enhancement of phagocytic ability

Active Publication Date: 2020-03-03
BEIJING UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] For a long time, the research on traditional Chinese medicine has mainly focused on secondary metabolites, and the possible pharmacological effects of proteins are hardly known.

Method used

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  • New Use of Dendrobium Polypeptide
  • New Use of Dendrobium Polypeptide
  • New Use of Dendrobium Polypeptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The extraction of embodiment 1 protein

[0020] Mix 25g of dendrobium powder with distilled water, leaching on a magnetic stirrer at room temperature for 3 hours, and centrifuge at 3000r / min for 5 minutes after gauze primary filtration, take the supernatant, dialyze, and freeze-dry to obtain water-soluble protein;

[0021] Add 0.1 mol / L sodium chloride solution to the dendrobium medicinal residue extracted with distilled water, mix well, stir and extract at room temperature for 3 hours, and centrifuge at 3000 r / min for 5 minutes after primary filtration with gauze, take the supernatant, dialyze to a certain volume, Freeze-drying to obtain salt-soluble protein;

[0022] Mix 25g of dendrobium powder with 75% ethanol solution, leaching on a magnetic stirrer at room temperature for 3 hours, and centrifuging at 3000r / min for 5 minutes after gauze primary filtration, take the supernatant, and freeze-dry to obtain alcohol-soluble protein;

[0023] Add 0.3% sodium hydroxide so...

Embodiment 2-5

[0025] The extraction of embodiment 2-5 protein

[0026] Take the 40-mesh Dendrobium chrysalis powder (after extraction with 75% ethanol), extract for different times and times with different concentrations of sodium hydroxide solution according to a certain solid-liquid ratio, then centrifuge at 3000r / min for 15min, and take The supernatant was dialyzed to obtain protein precipitation, dialyzed, and vacuum-dried to obtain protein dry powder. The extraction parameters and extraction yields are shown in Table 1.

[0027] Table 1 Extraction yield of different parameters

[0028]

Embodiment 6-10

[0029] Embodiment 6-10 activated carbon decolorization

[0030] Get 100ml of the protein solution prepared in Example 5, add different amounts of activated carbon for decolorization, and control the temperature, pH and time to measure the decolorization rate and protein loss rate. See Table 2.

[0031] It can be seen from Table 2 that in Example 8, the dosage and temperature of activated carbon are set to 1.2g and 45°C, the pH and decolorization time are set to 7.0 and 30min, and the decolorization rate is high and the protein loss is small.

[0032] Table 2 Activated carbon decolorization

[0033]

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PUM

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Abstract

The invention provides application of Dendrobium polypeptide in resisting oxidation and improving immunity. The method of the invention is adopted to fully utilize herb residues remaining in the actual production of Dendrobium to prepare Dendrobium polypeptide with good activity, and helps improve the additional utilization rate of Dendrobium chrysotoxum Lindl.

Description

technical field [0001] The invention relates to a new application of dendrobium polypeptide, which belongs to the field of traditional Chinese medicine. Background technique [0002] For a long time, the research on traditional Chinese medicine has mainly focused on secondary metabolites, and the possible pharmacological effects of proteins are hardly known. With the development and maturity of biological science and technology, the research on proteins and their peptides in traditional Chinese medicine has gradually received more attention, and may become a new direction in the research of traditional Chinese medicine and new drugs. The traditional theory holds that the human body absorbs protein mainly in the form of amino acids. The latest research has found that after enzymatic hydrolysis of the digestive tract, protein is mainly absorbed in the form of small molecular polypeptides, which are easier to be absorbed and utilized by the body than free amino acids. Therefo...

Claims

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Application Information

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IPC IPC(8): A61K38/01A61P39/06A61P37/04A61P17/00A61K8/64A61Q19/08A61Q19/00A61Q19/02A23L33/18
CPCA61K38/011A61P39/06A61P37/04A61P17/00A61K8/645A61Q19/08A61Q19/00A61Q19/02A23L33/18A23V2002/00A23V2200/318A23V2250/55A23V2200/324Y02A50/30
Inventor 孙志蓉陈龙曾宇馨祝天添
Owner BEIJING UNIV OF CHINESE MEDICINE
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