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Method for establishing fingerprints of related characteristics of chemical small molecule components of dendrobium chrysotoxum

A fingerprint, drumstick Dendrobium technology, applied in the field of fingerprint analysis and modeling, can solve the problems of high instrument requirements, inability to accurately detect macromolecular components, inability to accurately evaluate the quality of Dendrobium, and achieve strong pertinence and applicability, Realize the effect of correlation analysis

Active Publication Date: 2018-06-26
蓝标一成(海南)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In addition, in the existing Dendrobium detection standards, the polysaccharides and other macromolecular medicinal components are detected. However, the detection of polysaccharides and other macromolecular components requires high technology and equipment requirements, and the existing domestic instruments and equipment are usually unable to accurately detect macromolecules. Molecular composition, unable to accurately evaluate the quality of Dendrobium

Method used

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  • Method for establishing fingerprints of related characteristics of chemical small molecule components of dendrobium chrysotoxum
  • Method for establishing fingerprints of related characteristics of chemical small molecule components of dendrobium chrysotoxum
  • Method for establishing fingerprints of related characteristics of chemical small molecule components of dendrobium chrysotoxum

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] The extraction method of embodiment 1 Dendrobium drumstick

[0052] Take the dried sample of Dendrobium chrysalis, pulverize it with a pulverizer, pass through a pharmacopoeia sieve (aperture 0.335mm), accurately weigh 1.000g of Dendrobium powder (the weighing error cannot exceed 0.2%), place it in a 100ml Erlenmeyer flask, add 50mL of 75 % Methanol (V 水 :V 甲醇 =25:75), took out after ultrasonic 30min at room temperature, filtered, and the filtrate was concentrated to dryness with 75% methanol solvent (V 水 :V 甲醇 =25:75), and finally transferred to a 10ml volumetric flask to constant volume, shaken up, and filtered with a 0.45 μm microporous membrane to obtain a Dendrobium chrysanthemum sample solution.

Embodiment 2

[0053] The chromatographic detection method of embodiment 2 Dendrobium drumstick extract

[0054] 1. Sample extraction and processing method for the determination of small molecule components of Dendrobium drumsticks:

[0055] Take 1.00g of the powder of this product (passed through a No. 3 sieve), accurately weigh it, put it in a 100ml volumetric flask, add 50ml of methanol-water (75:25) accurately, ultrasonicate (power 250W, frequency 40kHz) for 30 minutes, let cool, After filtration, the filtrate was concentrated to dryness by rotary evaporation, dissolved in 5 ml of methanol-water (75:25), the supernatant was passed through a 0.45 μm microporous membrane, and the subsequent filtrate was obtained.

[0056] 2. Preparation of reference solution

[0057] Accurately weigh 4.10 mg of saffrontoside and 4.08 mg of naringenin respectively, place them in 10 ml volumetric flasks, add 75% methanol to dissolve and dilute, shake well, and use them as stock solutions. Refrigerate at 4°...

Embodiment 3

[0069] Embodiment 3 Establishment of the correlation characteristic fingerprint spectrum with schaffertoside in Dendrobium chrysanthemum

[0070] 1. Dendrobium sample solution preparation

[0071] Take the dry sample of Dendrobium, pulverize it with a pulverizer, pass through a pharmacopoeia sieve (aperture 0.335mm), accurately weigh 1.000g of Dendrobium powder (the weighing error cannot exceed 0.2%), place it in a 100ml Erlenmeyer flask, add 50mL of 75% methanol ( V 水 :V 甲醇 =25:75), took out after ultrasonic 30min at room temperature, filtered, and the filtrate was concentrated to dryness with 75% methanol solvent (V 水 :V 甲醇 =25:75), and finally transferred to a 10ml volumetric flask to constant volume, shaken up, and filtered through a 0.45μm microporous membrane to obtain the product. Table 2 is the obtained linear relationship of the reference substance schaftoside.

[0072] Table 2 The linear relationship table of the reference substance Schaftoside

[0073]

[0...

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Abstract

The invention discloses a method for establishing fingerprints of related characteristics of chemical small molecule components of dendrobium chrysotoxum. The method comprises: 1) respectively performing chromatographic detection on samples having a sample size of n, and obtaining test data with chemical small molecule components schaftoside and / or naringenin as reference components; 2) respectively performing fingerprint detection on the samples to obtain a peak area value of the fingerprint of all chemical components of dendrobium chrysotoxum; 3) taking the component content value of schaftoside and / or naringenin in chromatographic data as a response variable, taking a peak area value of other components in the fingerprint as an independent variable to establish an analytical model, andestablishing a linear regression model of the small molecular components and the fingerprints; 4) characterizing the contribution degree of the independent variable to regression modeling through a variable correlation coefficient index, and establishing the fingerprints of the related characteristics of the chemical small molecule components. According to the method, correlation analysis of the chemical small molecule component content is achieved, and the quality of the dendrobium chrysotoxum medicinal material can be effectively identified, controlled and evaluated.

Description

technical field [0001] The invention belongs to the field of detection and analysis of traditional Chinese medicine components, and in particular relates to a method for analyzing and modeling the fingerprint of Dendrobium drumsticks. Background technique [0002] Dendrobium is a commonly used tonic Chinese medicine, mainly for the Dendrobium genus. Dendrobium is the largest genus of Orchidaceae, including Dendrobium candidum, Dendrobium nobile, Dendrobium dendrobii, Dendrobium drumstick and other varieties. There are more than 1,100 species of Dendrobium in the world, of which 76 are found in my country. Dendrobium has a long history of medicinal use. As early as in ancient times, Dendrobium was listed as a nourishing top grade in "Shen Nong's Materia Medica". For a long time, with the development of the times, Dendrobium has been regarded as a precious Chinese herbal medicine with very important nourishing effects. Dendrobium is clinically used to treat a variety of dis...

Claims

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Application Information

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IPC IPC(8): G01N30/86
CPCG01N30/8686
Inventor 邵自星赵田
Owner 蓝标一成(海南)生物科技有限公司
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