The invention discloses a high-density pleurotus eryngii liquid strain and a fermentation method thereof, and belongs to the technical field of pleurotus eryngii cultivation. Scientific compounding is carried out on Chinese herb extracts capable of improving the cold resistance, thermal stress, immunity and infectious microbe resisting capacity of the strain in culture media of liquid seeds, shake flask seeds, seeding tank seeds and fermentation cylinder liquid strains at all stages, meanwhile, scientific compounding is carried out on wood bit powder with raw material adaptability and antifoaming agent functions, a supplemental inoculation and subsection temperature varying fermentation mode is adopted, the cell age of the strain and the multiplication generation gap are shortened, coated chitosan with the special function is fed in good time, the pleurotus eryngii liquid strain with thick and solid fermentation mycelia, high activity, the small mycelium pellet diameter, large density and even distribution is cultured, the mycelium pellet density is 4.5-5.5*105 per liter, the diameter of the mycelium pellet is 0.5 mm to 0.7 mm, the dry weight of the mycelium pellet is 90 g/L to 100 g/L, germinating time is 4 h to 5 h, and exopolysaccharides is 3.0 g/L to 3.5 g/L.