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Liquid strain fermentation culture medium

A technology of fermentation medium and liquid strains, which is applied in the fields of fungi, horticulture, botanical equipment and methods, etc. It can solve the problems of short fermentation period and small diameter of mycelium balls, and achieve small diameter of mycelium balls and high efficiency of fermentation bacteria. Silk thick, evenly distributed effect

Inactive Publication Date: 2015-10-21
TIANJIN ZHONGTIAN JINGKE SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem solved by the present invention is to overcome the defects of the existing Pleurotus eryngii liquid strains prepared by aerated fermentation, and the scientific compounding in the culture medium of each stage of the strain cultivation process can improve the strain resistance to cold, heat stress, and anti-coldness. Disease and immune Chinese herbal medicine extracts and adaptive raw material sawdust powder with defoaming function to prepare a fermented mycelia that can make the fermented mycelia thick, high in activity, small in diameter, high in density, evenly distributed, and short in fermentation cycle Pleurotus eryngii liquid strain fermentation medium

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1 raw material preparation

[0050] 1. Preparation of Chinese herbal medicine extract:

[0051] The preparation method of the Chinese herbal medicine extract comprises the steps of: weighing 30 parts of astragalus, 30 parts of dried ginger, 30 parts of tangerine peel, 25 parts of bezoar, 25 parts of paeonol, 20 parts of licorice, and 18 parts of anemarrhena in parts by weight. 18 parts of honeysuckle, 17 parts of Houttuynia cordata, 15 parts of Chuanxiong, and 10 parts of angelica; put them in an ultrasonic cleaning machine filled with 0.2% sodium bicarbonate solution at 200W and 30KHz for 12min, drain, and crush the above-mentioned Chinese herbal medicines into granular The diameter is less than 2 mm, put it in a container and mix it evenly and add 5 times the weight of water to get the mixed material, control the temperature at 80°C for 3 hours, then cool down to 45°C, adjust the pH value to 4 with lactic acid, and carry out under the condition of power 200...

Embodiment 2

[0059] A kind of method utilizing fermentation medium to prepare liquid strain, comprises the steps:

[0060] 1) Take the preserved Pleurotus eryngii slant strain 0.2cm 2 Inoculate 2 pieces of the strain block of the above-mentioned bacteria on the plate medium for activation, and culture at a constant temperature of 25°C for 10 days, and activate twice in this way to obtain plate seeds;

[0061] 2) Use a puncher with a diameter of 0.5 cm to intercept the flat seed 0.2 cm 2 3 pieces of the strain block were inserted into a 250mL Erlenmeyer flask, and the amount of liquid seed culture medium was 100mL. They were cultured at a constant temperature of 25°C for 2 days, then placed in a constant temperature shaker, and shaken at 25°C and 100r / min for 3 days. liquid seeds;

[0062] 3) The liquid seeds were inoculated in a 500 mL shake flask with an inoculum amount of 10%, and the liquid volume of the shake flask culture medium was 250 mL, and cultured at 25° C. and 150 r / min at a ...

Embodiment 3

[0073] A kind of method utilizing fermentation medium to prepare liquid strain, comprises the steps:

[0074] Step 1) to 4) with embodiment 2;

[0075] 5) First inoculate the seeds of the seed tank with 8% inoculum amount in a 30L fermenter, the fermenter medium capacity is 15L, and ferment at 30°C at a constant temperature of 3L / min for 12h, and then cool down at a rate of 0.3°C / min to 18°C, add 10g coated chitosan and 900mL seed tank seeds into the fermenter, ferment at constant temperature for 10h under the condition of ventilation rate 6L / min, and finally raise the temperature to 24°C at the rate of 0.2°C / min Ferment at constant temperature for 45 hours under the condition of 1 min to obtain the liquid strain;

[0076] Described plate medium composition is the same as embodiment 2;

[0077] The liquid seed medium consists of: glucose 30g / L, yeast extract juice 5g / L, peptone 2g / L, agar 2g / L, Chinese herbal medicine extract 0.1g / L, potassium dihydrogen phosphate 0.5g / L, ma...

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Abstract

The invention discloses a liquid strain fermentation culture medium, and belongs to the technical field of pleurotus eryngii cultivation. Chinese herb extracts capable of improving cold resistance, heat irritability resistance, immunity and bacterium-resisting capacity of the strain are scientifically compounded in culture mediums for liquid seeds, shaking flask seeds, seeding tank seeds and fermentation tank liquid strains in all stages, and meanwhile wood powder which has the feedstock adaptability and has the function of an antifoaming agent is scientifically compounded; manners of supplemental inoculation and segmentation variable temperature fermentation are adopted, and therefore the difference between the strain cell age and the multiplication algebra is shortened; and packaged chitosan with a special function is properly fed, and the pleurotus eryngii liquid strain with the thick and solid fermentative hyphae, high activity, small mycelium pellet diameter, large density and uniform distribution is cultured. The density of the mycelium pellets ranges from 4.5*10<5>piece / L to 5.5*10<5>piece / L, the diameter of the mycelium pellets ranges from 0.5mm to 0.7mm, the dry weight of the mycelium pellets ranges from 90g / L to 100g / L, the germination time ranges from 4 hours to 5 hours, and the exopolysaccharide ranges from 3.0g / L to 3.5g / L.

Description

technical field [0001] The invention relates to the technical field of Pleurotus eryngii cultivation, in particular to a liquid strain fermentation medium. Background technique [0002] Pleurotus eryngii (PleurotuseryngiiQuel.), also known as Pleurotus eryngii, has the advantages of thick flesh and crisp texture, especially its dense, firm, milky white and refreshing stipe. It is called "king of oyster mushrooms" and "scallop mushrooms". , with almond fragrance and abalone taste, suitable for fresh-keeping and processing, and is deeply loved by people. It is rich in nutrients, rich in protein, carbohydrates, vitamins and minerals such as calcium, magnesium, copper, zinc, etc., which can improve human immune function. It has the effects of anti-cancer, lowering blood fat, moisturizing the stomach and beautifying the human body, and has extremely high cultivation value and commercial value. [0003] At present, the cultivation of Pleurotus eryngii at home and abroad is domina...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01G1/04
Inventor 李建树
Owner TIANJIN ZHONGTIAN JINGKE SCI & TECH
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