A method for judging and detecting the similarity of pure Dendrobium chrysanthemum

Abstract

The invention relates to a method for judging and detecting the similarity of pure species of Dendrobium chrysalis. The establishment process of the judging method is as follows: S1: collect samples of Dendrobium chrysalis that are consistent with the conclusion of gene sequencing, and measure the total length of each sample; S2: The normality test is carried out on the total length of the sample; S3: standard interval: if the result obtained in step S2 obeys the normal distribution, then the 95% confidence interval of the mean and 95% of the standard deviation are obtained according to the general calculation formula of the normal distribution. % confidence interval; this confidence interval can be used as a standard range to identify the purity of unknown samples. The judgment standard established according to the data actually collected is: the 95% confidence interval of the mean value and the 95% confidence interval of the standard deviation are respectively: (32.6567, 36.2100) and (5.8296, 8.3883), the tested Dendrobium chrysalis sample only needs to match the Compare intervals. The present invention can identify the purity of the species of Dendrobium chrysalis through its morphology-related features, so as to simply judge the value or existence of the artificially planted Dendrobium chrysalis; the identification accuracy is high, and it has important practical significance .

Description

technical field [0001] The invention relates to the fields of medicine and biology, in particular to a method for judging and detecting the similarity of pure Dendrobium chrysalis, that is, a method for establishing the correlation between the morphological characteristics of Dendrobium chrysalis and the conclusion of gene sequencing, and the species of Dendrobium chrysalis to be tested Identification method of purity, etc. Background technique [0002] Dendrobium is a commonly used tonic Chinese medicine, mainly for the Dendrobium genus. The genus Dendrobium is the largest genus in the Orchidaceae plant, including Dendrobium meihua, Dendrobium fringe, Dendrobium officinale, Dendrobium nobile, Dendrobium dendrobii, Dendrobium drumstick and other varieties. There are more than 1,100 species of Dendrobium in the world, of which nearly 100 species are found in my country. Dendrobium has a long history of medicinal use. It was listed as a top-grade nourishing product in "Shen ...

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Problems solved by technology

[0003] However, due to long-term unrestrained excavation and irrational use of Dendrobium, its wild resources are decreasing day by day, and artificial planting is gradually increasing, and it has even become the main source of Dendrobium chrysalis.

However, long-term artificial planting has also brought about false ones and shoddy ones for Dendrobium chrysalis. This is because ① artificial planting has changed the growth environment of wild Dendrobium chrysalis; , the application of pesticides, the emergence of new disease types, etc. are artificially imposed on Dendrobium chrysalis; ③And because there are many varieties of Dendrobium, the hybridization between the varieties makes the traits of its close relatives cross; ④Others are uncontrollable Or unmeasurable factors, in summary, the medicinal ingredients of some artificially planted Dendrobium chrysalis have changed, and even important medicinal ingredients have disappeared. The gene sequence is also substantially different from the original wild gene sequence

Once the medicinal value of these Dendrobium chrysalis weakens or disappears, and the field continues to use it without knowing it, the consequences will be very serious. When people do not know it, Dendrobium chrysalis will disappear from the medical field, the consequences are more serious

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