Establishment of method of judging Dendrobium devonianum Paxt pure-species closeness, judgment standard and judgment method

Abstract

The invention relates to establishment of a method of judging Dendrobium devonianum Paxt pure-species closeness, a judgment standard and the judgment method. An establishment process includes: S1, collecting samples, and determining stem third-internode-length of each sample; S2, carrying out a normality test; and S3, obtaining a standard interval, wherein if an obtained result obeys normal distribution, a confidence interval is calculated according to an overall formula of the normal distribution, and can be used as a standard range of identifying purity of an unknown sample. The judgment standard is that a 95% confidence interval of a mean value and a 95% confidence interval of standard deviation are respectively (28.7833, 30.5697) and (4.7839, 6.0569). The detected Dendrobium devonianumPaxt sample only needs to be compared with the intervals. The method can identify the species purity of Dendrobium devonianum Paxt through a morphologically related feature of Dendrobium devonianum Paxt, and can thus simply judge a value or presence of artificially planted Dendrobium devonianum Paxt; and is high in accuracy of identification, and has important practical significance.

Description

technical field [0001] The present invention relates to the fields of medicine and biology, and in particular to the establishment of a method for judging the similarity of Dendrobium dendrobium purebred, a judgment standard and a judgment method, that is, a method for establishing the correlation between the morphological characteristics of Dendrobium dendrobium and the conclusion of gene sequencing and The identification method of the purity of the tested species of Dendrobium dendrobii, etc. Background technique [0002] Dendrobium is a commonly used tonic Chinese medicine, mainly for the Dendrobium genus. Dendrobium is the largest genus of Orchidaceae, including Dendrobium dendrobium, Dendrobium fringe, Dendrobium officinale, Dendrobium nobile, Dendrobium dendrobii, Dendrobium drumstick and other varieties. There are more than 1,100 species of Dendrobium in the world, of which nearly 100 species are found in my country. Dendrobium has a long history of medicinal use. I...

AI Technical Summary

Problems solved by technology

[0003] However, due to long-term unrestrained excavation and irrational use of Dendrobium, its wild resources are decreasing day by day, and artificial planting is gradually increasing, and it has even become the main source of Dendrobium dendrobium.

However, the long-term artificial planting has also brought about false ones and shoddy ones for Dendrobium dendrobii. This is because ① artificial planting has changed the growth environment of wild Dendrobium dendrobii; , the application of pesticides, the emergence of new disease species, etc. are artificially imposed on Dendrobium dendrobium; ③And because there are many varieties of Dendrobium, the hybridization between the varieties makes the traits of its close relatives cross; ④Others are uncontrollable Or unmeasurable factors, in summary, the medicinal ingredients of some artificially planted Dendrobium nodule have changed, and even important medicinal ingredients have disappeared. The gene sequence is also substantially different from the original wild gene sequence

Images