Tissue culture quick reproduction method for dendrobium chrysotoxum lindl bud tips

A technology for tissue culture and Dendrobium Dendrobium, which is applied in the field of rapid propagation of Dendrobium Dendrobium sprout tip tissue culture, can solve problems that hinder the development and utilization of Dendrobium Dendrobium Dendrobium, the protection of native species of Dendrobium Dendrobium, the high degree of separation of the characters of the descendants of Dendrobium Dendrobium, and the difficulty in solving the problems. Realize the problems of plants with the same traits, and achieve the effect of less investment, short cycle and easy operation

Inactive Publication Date: 2011-10-19
广东省农业科学院花卉研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The offspring of Dendrobium chrysalis cultivated by this technology have a high degree of separation of traits and large differences among individuals, making it difficult to achieve plants with consistent traits, which hinders the protection of the original species of Dendrobium chrysalis and the development and utilization of Dendrobium chrysalis in the flower market

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Select 5-8 cm long young shoots, remove the packs, rinse them with tap water and cut them into two sections, soak them in 70% alcohol for 15 seconds on a clean bench, and then sterilize them with 0.1% mercury solution After washing 5 times with sterile water for 8 minutes, absorb the residual water with sterile filter paper, and cut 1-2 cm long stems with lateral bud tips to inoculate the induction medium with 1 / 2MS+6-BA(6-benzyl Amino adenine) 0.5㎎ / L+AD (Adenine) 1.0㎎ / L+AgNO 3 5.0 ㎎ / L +10.0% coconut juice+5.0g / L activated carbon, light intensity 1500~2000lx, light 10 hours / day, temperature 25~28℃. After 40 to 55 days of cultivation, new shoots are formed and grow. The clumping buds obtained by cultivating the bud tips are cut and transferred to the clumping bud propagation medium 1 / 2MS+6-BA (6-benzylamino adenine) 1.0㎎ / L+AD (adenine) 0.5㎎ / L +AgNO 3 5.0㎎ / L +10.0% coconut juice+5.0g / L activated carbon, under the conditions of light intensity 1500~2000lx, light for 10 h...

Embodiment 2

[0038] Select 5-8 cm long young shoots, remove the packs, rinse them with tap water and cut them into two sections, soak them in 70% alcohol for 30 seconds on a clean bench, and then sterilize them with 0.1% mercury solution After washing with sterile water for 6 times for 10 minutes, absorb the residual water with sterile filter paper, and cut 1-2 cm long stems with lateral bud tips to inoculate the induction medium with 1 / 2MS+6-BA(6-benzyl Amino adenine) 0.75㎎ / L+AD (Adenine) 1.25㎎ / L+AgNO 3 7.5 ㎎ / L +15.0% coconut water+7.5g / L activated carbon, light intensity 1500~2000lx, light 11 hours / day, temperature 25~28℃. After 40 to 55 days of cultivation, new shoots are formed and grow. The clumping buds obtained by cultivating the bud tips are cut and transferred to the clumping bud propagation medium 1 / 2MS+6-BA (6-benzylamino adenine) 1.5㎎ / L+AD (adenine) 0.75㎎ / L +AgNO 3 7.5㎎ / L +15.0% coconut juice+7.5g / L activated carbon, under the conditions of light intensity 1500~2000lx, light ...

Embodiment 3

[0040] Select 5-8 cm long young shoots, remove the packs, rinse them with tap water and cut them into two sections, soak them in 70% alcohol for 45 seconds on an ultra-clean workbench, and then sterilize them with 0.1% mercury solution After rinsing with sterile water for 8 times for 12 minutes, use sterile filter paper to absorb the residual water, and cut 1-2 cm long stems with lateral bud tips to inoculate the induction medium with 1 / 2MS+6-BA(6-benzyl Amino adenine) 1.0㎎ / L+AD (Adenine) 1.5㎎ / L+AgNO 3 10.0 ㎎ / L +20.0% coconut juice + 10.0g / L activated carbon, light intensity 1500~2000lx, light 12 hours / day, temperature 25~28℃. After 40 to 55 days of cultivation, new shoots are formed and grow. The clump buds obtained by cultivating the bud tips are cut out and transferred to the clump bud propagation medium 1 / 2MS+6-BA(6-benzylamino adenine) 3.0㎎ / L+AD(adenine) 1.0㎎ / L +AgNO 3 10.0㎎ / L +20.0% coconut juice + 10.0g / L activated carbon, at light intensity of 1500~2000lx, light for ...

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Abstract

The invention provides a tissue culture quick reproduction method for dendrobium chrysotoxum lindl bud tips, which comprises the following steps of: performing induction culture of buds; performing enrichment culture of tufted buds; performing subculture of the tufted buds; and performing rooting culture to obtain a complete plant, and hardening seedling outside a bottle and transplanting. The method is easy to operate and low in production cost, environment pollution is avoided, and large-scale production can be realized. Dendrobium chrysotoxum lindl seedlings cultured by the method are stable in inheritable characters, keep the characteristics of parents and have the advantages of invariance property, small investment, high yield and short period.

Description

Technical field [0001] The invention belongs to the technical field of tissue culture rapid propagation, and specifically relates to a method for rapid propagation of Dendrobium Dendrobium bud tip tissue culture. Background technique [0002] Drumstick Dendrobium ( Dendrobium chrysanthum Wall.ex Lindl.) is a plant of the genus Orchidaceae Dendrobium, also known as Dendrobium chinense. It is mainly distributed in my country's Yunnan, Guangxi, Guizhou and other provinces, as well as Malaysia, India, and Myanmar. According to the "Chinese Pharmacopoeia" record: its taste is light, slightly cold in nature, has the effects of nourishing yin, nourishing stomach, clearing away heat and detoxification; clinically, it is mostly used to treat chronic pharyngitis, ophthalmological diseases, and thrombo-occlusive diseases, with obvious effects; chemical composition And pharmacological activity studies have shown that Dendrobium Drumstick contains anti-tumor active ingredients such as Drums...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈和明吕复兵朱根发操君喜孙映波
Owner 广东省农业科学院花卉研究所
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