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This invention discloses a method for preparing recombinant astaxanthin vesicles derived from Haematococcus pluvialis with gut-protective and lipid-regulating effects, belonging to the field of bioactive substance delivery. This system uses Haematococcus pluvialis as raw material, without adding any exogenous carriers, directly extracting natural astaxanthin and algal lipids, which are then recombined and self-assembled to form a stable nano-delivery system. Algal lipids serve as the carrier framework, astaxanthin as the active ingredient, phospholipids participate in the formation of the lipid bilayer, and astaxanthin is embedded in the hydrophobic region, achieving efficient delivery and oral administration. This invention utilizes the synergistic effect of the algal components themselves to solve the problems of poor water solubility and low bioavailability of astaxanthin. Combining the lipid-regulating effects of algal lipids with the gut-protective and antioxidant effects of astaxanthin, it achieves a three-in-one effect of "delivery, protection, gut protection, and lipid regulation." It can be used in functional foods, health products, and pharmaceutical preparations to improve intestinal microecology and regulate lipid metabolism, possessing advantages such as natural safety, simple process, low cost, and broad application prospects.