The invention relates to a
plant cell scale
moisture absorption deformation determination method based on a freezing
scanning electron microscope. The method comprises the steps of sample pretreatment,
moisture absorption treatment,
cell scale
image acquisition in a water absorption state,
cell scale
image acquisition in a water loss state and
moisture absorption deformation determination. According to the method, the freezing
scanning electron microscope is introduced, and images in a rehydration state and a water loss state are obtained in sequence, so that a cell scale
moisture absorption deformation result of the phleborus crispus is obtained; the method can solve the problem that a conventional instrument is difficult to accurately capture
microstructure changes in water loss and rehydration states due to rapid changes of water-driven plants, especially
phlomis rugosa tissues.