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Process for extracting erianin from Dendrobium chrysotoxum Lindl

A technology of Dendrobium Dendrobium and Erianin, applied in the directions of ether separation/purification, ether preparation, organic chemistry, etc., can solve the problems of high technical requirements, serious environmental pollution, large amount of organic solvent, etc., and achieves simple extraction method and separation. Good effect and high product purity

Inactive Publication Date: 2011-02-16
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In the 1990s, Chinese scholars isolated and extracted Erianin from Dendrobium, and the extraction method was to use chloroform or benzene as the extraction solvent and extract it with ultrasonic treatment for 30 minutes. Shaped Erianin crystalline powder; the shortcoming of this method is: the organic solvent consumption is big (more than 10 times that is the dendrobium raw material), and the cost is high, and the time is long, and serious to environmental pollution
Chinese patent application 03115752.1 discloses a method for extracting Erianin from Dendrobium, including the application of supercritical CO 2 Extraction and column chromatography use absolute ethanol, methanol, acetone, etc. as entrainers, and CO 2 Equivalent extraction medium extracts Erianin, an anti-tumor component from the orchid plant Dendrobium, and then uses petroleum ether: ethyl acetate solution as the eluent to perform silica gel column chromatography separation and recrystallization on the obtained crude extract. Refined extract; because the above-mentioned extraction method has high technical requirements, it is not conducive to large-scale production

Method used

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  • Process for extracting erianin from Dendrobium chrysotoxum Lindl

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Grind the dried product of Dendrobium chrysalis purchased from Yunnan, take 1kg and soak it in ethyl acetate with 6 times the weight of Dendrobium chrysalis at room temperature for 4 days, filter under normal pressure, collect the ethyl acetate extract A, repeat 2 times, and combine the extract A . The medicinal dregs after the second extraction were extracted with 3 times the amount of 90% ethanol in a 400W microwave for 10 minutes, extracted 3 times, filtered at normal pressure, collected the ethanol extracts, combined, and reduced pressure at 65 ° C (vacuum degree is 0.09 Mpa) was concentrated to obtain ethanol extract, extracted three times with ethyl acetate equivalent to 2 times the amount of extract, combined ethyl acetate extract B, combined two parts of ethyl acetate extract A and B, and vacuumed at 80°C (vacuum degree is 0.095Mpa) concentrating to obtain the extract, and naturally evaporated to dryness to obtain the total extract of ethyl acetate.

[0021] Th...

Embodiment 2

[0023] Grind the dried product of Dendrobium chrysalis purchased from Yunnan, take 2.5kg and soak it in ethyl acetate with 7 times the weight of Dendrobium chrysalis at room temperature for 6 days, filter under normal pressure, collect the ethyl acetate extract A, repeat 3 times, and combine the extracts a. The medicinal dregs after the third extraction were extracted with 4 times the amount of 95% ethanol in a 350W microwave for 15 minutes, extracted 3 times, filtered at normal pressure, collected the ethanol extracts, combined, and reduced pressure at 62 ° C (vacuum degree is 0.085 Mpa) was concentrated to obtain ethanol extract, extracted 4 times with ethyl acetate equivalent to 3 times the amount of extract, combined ethyl acetate extract B, combined two parts of ethyl acetate extract A and B, and vacuumed at 85°C (vacuum degree is 0.095Mpa) concentrating to obtain the extract, and naturally evaporated to dryness to obtain the total extract of ethyl acetate.

[0024] The ...

Embodiment 3

[0026] Grind the dried product of Dendrobium chrysalis purchased from Yunnan, take 5 kg and soak it in ethyl acetate with 8 times the weight of Dendrobium chrysalis at room temperature for 5 days, filter under normal pressure, collect the ethyl acetate extract A, repeat 2 times, and combine the extract A . The medicinal dregs after the second extraction were extracted with 4 times the amount of 90% ethanol in a 350W microwave for 20 minutes, extracted twice, filtered at normal pressure, collected the ethanol extracts, combined, and reduced pressure at 60°C (vacuum degree is 0.090 Mpa) was concentrated to obtain ethanol extract, extracted 3 times with ethyl acetate equivalent to 3 times the amount of extract, combined ethyl acetate extract B, combined two parts of ethyl acetate extract A and B, and vacuumed at 90°C (vacuum degree is 0.095Mpa) concentrating to obtain the extract, and naturally evaporated to dryness to obtain the total extract of ethyl acetate.

[0027] Mix the ...

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Abstract

The invention relates to a process for extracting erianin from Dendrobium chrysotoxum Lindl. The process is characterized by comprising the following steps of: preparing a Dendrobium chrysotoxum Lindl ethyl acetate extractive by methods of dipping extraction and microwave-assisted extraction; mixing with aluminum oxide and carrying out column chromatography; eluting with an organic solvent and carrying out chromatography and detecting by a silica gel thin layer; collecting the same fraction as Rf=0.2-0.6 and recovering the reagent; concentrating and crystallizing; recrystallizing with methanol or acetone; and drying to obtain erianin. The extracted compound opens a new approach for developing and applying a new generation of natural source anti-tumor medicaments.

Description

Technical field: [0001] The invention belongs to the field of natural plant extraction, and relates to a process for extracting erianin from Dendrobium pachyrhiza. Background technique: [0002] Erianin, Chinese system name: 3-hydroxy-4,3',4',5'-tetramethoxybibenzyl; molecular formula: C 18 h 22 o 5 ; Molecular structural formula is as follows: [0003] [0004] Erianin is a bibenzyl compound, easily soluble in ethanol, chloroform, acetone, methanol and other organic solvents, and very slightly soluble in water. [0005] Hong Wei et al. (The effect of erianin on telomerase activity in gastric cancer cell SGC-7901. Hong Wei, Guo Yong, Ma Shenglin, et al. Zhejiang Practical Medicine, 2009, 14(3): 181-182.) found that erianin Lansu can significantly inhibit the proliferation of gastric cancer cell SGC-7901 in a concentration-dependent manner, with a 48-hour IC 50 It is 0.056μg / ml. The study found that erianin can quickly inhibit the telomerase activity of gastric cancer...

Claims

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Application Information

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IPC IPC(8): C07C43/23C07C41/36
Inventor 刘东锋郭琴杨成东
Owner NANJING ZELANG MEDICAL TECH
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