The invention discloses a graphene hygienic disinfection wet tissue, which comprises a graphene spunlace non-woven fabric and a wet tissue liquid, and the wet tissue liquid comprises ultrapure water and isopropanol, wherein the ultrapure water accounts for 25%-60% of the weight of the wet tissue liquid, the isopropanol accounts for 40%-75% of the weight of the wet tissue liquid, and the viscose content of the graphene spunlace non-woven fabric is 10%-50%. According to the invention, because of strong permeability and sterilization and disinfection effects of isopropanol, the concentration of disinfection liquid can be reduced, a disinfectant does not need to be added, and the harm of chemical substances is avoided. The graphene has a sterilization effect, and three sterilization mechanismsof the graphene perform sterilization in a physical mode, so that the graphene has a very good sterilization effect on bacteria. The hygienic disinfection wet tissue does not contain irritant substances such as essence, does not have side effects on skin surface disinfection, has diverse uses, is convenient to carry and use, is rapid to volatilize without residues. The hygienic disinfection wet tissue of the invention has the advantages of simple production process, convenient operation and 99.9% of killing effect on Escherichia coli, Staphylococcus aureus, Candida albicans and the like.