The invention discloses a culture medium for
ganoderma sinense cultivation and a preparation method and application thereof, and belongs to the technical field of
edible fungus cultivation. The culture medium is prepared from the following main materials in percentage by weight: 13 to 20 percent of couch grass, 28 to 35 percent of arundo
fungus grass and 40 to 45 percent of dicranopteris pedata, and the following auxiliary materials in percentage by weight: 0.1 to 0.8 percent of brown
sugar, 0.8 to 1.8 percent of
gypsum powder, 0.7 to 1.8 percent of
urea, 0.2 to 1.2 percent of
monopotassium phosphate, 0.05 to 0.16 percent of shell
powder, 0.9 to 2.3 percent of
corn flour and 0.02 to 0.07 percent of phoenix
mushroom essence. The preparation method comprises the following steps: uniformly mixing the main material and the auxiliary material respectively, totally mixing, adjusting the
water content to 45-65% and the pH value to 5-7, bagging, sealing, and sterilizing by high-pressure steam to obtain the sterile culture medium. The culture medium is used for cultivation of
ganoderma sinense, the
biological efficiency can reach 4.98 + / -1.07%, the
polysaccharide content of sporocarp can reach 635 mg / 5 g, and the culture medium is obviously superior to a traditional coniferous tree
sawdust culture medium and a broad-leaved tree
sawdust culture medium. The culture medium for
ganoderma sinense cultivation is easy to obtain, simple and convenient in preparation process, capable of effectively increasing the yield of
ganoderma sinense and the content of main active ingredients and suitable for large-scale cultivation and application.