The invention discloses a
ganoderma lucidum cultivation method based on a
taxus chinensis substrate, which comprises the following steps: S1, substrate preparation: uniformly mixing the following components in percentage by weight: 20-35% of crushed
taxus chinensis branches and leaves, 10-20% of
taxus chinensis
bark extraction residues, 20-25% of
inonotus obliquus leavening, 10-15% of slow-release
nutrition bags, 10-20% of broad-leaved tree
sawdust and 5-10% of auxiliary materials to obtain a basic substrate; s2, substrate pretreatment: sequentially carrying out stepped enzymolysis, directional
fermentation and
microwave-assisted extraction treatment on the basic substrate to obtain a pretreated substrate; s3, cultivation management: putting the pretreated substrate into a cultivation container, inoculating
ganoderma lucidum strains in a laminated manner, and sequentially performing environment regulation and control in a
hypha growth stage, a sporocarp differentiation stage and a sporocarp growth stage to cultivate
ganoderma lucidum; and S4, harvesting: when white growth circles on the edges of the
ganoderma lucidum sporocarp disappear, pilei are uniform in color and spores start to be ejected, harvesting the sporocarp, and completing
ganoderma lucidum cultivation.