The invention discloses a traditional Chinese medicine composition for relieving uneasiness and supplementing qi after operation and a preparation method thereof. The traditional Chinese medicine composition for relieving uneasiness and supplementing qi after operation comprises the following materials: benzoin, lily bulb, cypraeae concha, catharanthus roseus, medulla junci, cecidien, flos albizzia, choerospondias axillaris, Edgeworthia chrysantha Lindl., oenanthe stolonifera, Swertia bifolia Batal, cactus, elm leaves, panax quinquefolium, stigma croci, rhodiola henryi, husked sorghum, yam, alpiniae oxyphyllae fructus, longan aril, rigescent hawksbeard root, lotus seed and wild panax pseudo-ginseng, and further comprises the following auxiliary materials: traditional Chinese medicine nutrient composite powder, bee honey and water soluble vitamin. The preparation method comprises the steps of extracting the effective ingredients of the raw materials to be prepared into extract powder, and preparing the extract powder together with the auxiliary materials into corresponding preparation formulations according to conventional pharmaceutical processes. The traditional Chinese medicine composition disclosed by the invention has a reasonable formula with the materials supplementing each other, has the effects of tranquilizing and allaying excitement, nourishing blood and supplementing qi, strengthening the spleen and replenishing qi, treating consumptive diseases, strengthening the waist and knees, strengthening the liver and stomach, replenishing essence and blood, strengthening tendons and muscles and nourishing heart and easing mind, enhances the immune function of bodies, has a remarkable treatment effect, and is especially suitable for patients of postoperative recovery.