41 results about "Clitocybe" patented technology

The invention relates to a method for preparing clitocybe maxima liquid strains by using yellow serofluid as a main raw material. The method comprises the follow steps: A, pretreatment of yellow serofluid: filtering and sub-packaging fresh yellow serofluid, then conducting pasteurization for 30 minutes at 62 DEG C, and reserving the sterilized yellow serofluid for later use; B, by using the yellow serofluid as the raw material and using waste molasses as a substrate, selecting and determining a liquid fermentation medium including 0.5-3.0 percent of waste molasses, 0.1 percent of MgSO4.7H2O, 0.2 percent of KH2PO4, 0.01g / L of VB1 (Vitamin B1), 0.05-1.5 percent of thickening agent, 0.2 percent of weed tree sawdust powder and the balance of pretreated yellow serofluid; regulating pH to 4.5-6.5; C, ensuring the liquid fermentation conditions as follows: pH is 4.5-6.5, the inoculum size is 10 percent, the temperature is 25-31 DEG C, the rotating speed of shaking table is 160r / min, and cultivating time is 49-120 hours; and D, inoculating peach oyster-cap fungus liquid strains obtained in the step C through fermentation into a barley seed substrate strain bottle with weed tree sawdust powder according to 10% of the inoculum size. The method fully utilizes nutrient contents of yellow serofluid and waste molasses and is beneficial to shortening the strain preparation cycle and production cycle of clitocybe maxima.