The invention discloses an external medicine for treating pain and regulating meridians and collaterals and a preparation method of the external medicine. The external medicine comprises Panax notoginseng, frankincense, myrrh, draconis sanguis, native copper, ground beeltle, safflower carthamus, asarum, angelica sinensis, peach kernel, radix paeoniae alba, notopterygium root, radix angelicae pubescentis, rheum officinale, sappan wood, radix clematidis, nux vomica, monkshood, radix aconiti kusnezoffii, Arisaema heterophyllum Blume, Anisodus acutangulus C. Y. Wu et C. Chen, Aristolochia fordiana, Kadsura coccinea, Zanthoxylum nitidum, stem and leaf of Illigera Khasiana c.B.Clarke, Gnetum montanum, asiatic toddalia root, ardisia crenata, radix cynanchi paniculati, aconitum brachypodum diels,Longtube Ground Ivy Herb, uncaria laevigata, Adhatoda ventricosa, cortex acanthopanacis, Neocinnamomum lecomtei Liou[N.hainanianum Allen], common jasminorange leaf and twig, common gendarussa herb, camphor, peppermint oil and garter snake. The invention has the beneficial effects that the external medicine is prepared from various Chinese herbal medicines, can be used for treating symptoms such aspain, numbness, soreness, distension, dizziness and itching caused by diseases or other reasons, also has a treatment effect on gynecology and andrology and can be used for conditioning the body andmobilizing the body function; and the external medicinal liquor does not injure the skin and has no side effect.