Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing extracting solution of gnetum montanum which can inhibit human liver cancer cell strain

A technology of human liver cancer cell line and liver cancer cell, which is applied in the field of preparation of the extract of Maima vine, can solve the problem of unclear anti-tumor effect, and achieve the effect of significant inhibitory effect, enriching plant resources, and simple extraction process.

Inactive Publication Date: 2006-02-15
SHANGHAI JIAO TONG UNIV
View PDF0 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Through the retrieval of prior art documents, Chen Hao and Lin Mao mentioned in the 8th issue of "Chinese Herbal Medicine" in 1999 "Research on the Chemical Components of Dayemeimateng" that the crude extract of Dayemaimateng was polymerized. Amide, silica gel as the stationary phase, water-ethanol, chloroform-methanol, methanol-water as the mobile phase for multiple column chromatography, 15 compounds were obtained, but whether the substances prepared by this method have anti-tumor effect is not yet clear

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Take by weighing 200 ℃ of pulverized vine powder after drying at 20 ℃ (fine powder of 40 mesh particle size), choose dehydrated alcohol: deionized water=7: 3 mixed solvent 2000ml, under 50 ℃, heating and refluxing extracts three times, each 2 Hours, the solvent was recovered by vacuum concentration at 45° C. to obtain 80.9 g of paste, which was dissolved in deionized water, extracted 6 times with equal volume of ethyl acetate, and concentrated to obtain 10.8 g of extract. Take 1 g of the substance, use deionized water-ethanol as the eluent, and perform column chromatography on polyamide (14 mesh) to obtain effective components, concentrate, and freeze-dry at -40°C to obtain 81.4 mg.

[0018] In vitro, 0.063 mg / ml of this component was stained with sulforhodamine B protein, and the inhibitory rate to human liver cancer cell line BEL-7402 was 87.0%.

Embodiment 2

[0020] Take by weighing 200g (fine powder of 40 order grain size) of the vine powder of vines that pulverizes after drying under 25 ℃, choose dehydrated alcohol: deionized water=7: 3 mixed solvent 2000ml, under 70 ℃, heat and reflux extraction three times, each time After 2 hours, the solvent was concentrated in vacuo at 45° C. to obtain 80.9 g of paste, dissolved in deionized water, extracted 6 times with equal volume of ethyl acetate, and concentrated to obtain 10.8 g of extract. Take 1 g of the substance, use deionized water-ethanol as the eluent, and perform column chromatography on polyamide (100 mesh) to obtain effective components, concentrate, and freeze-dry at -40°C to obtain 62.3 mg.

[0021] In vitro, 0.25 mg / ml of this component is stained with sulforhodamine B protein, and the inhibitory rate to human liver cancer cell line BEL-7402 is 63.7%.

Embodiment 3

[0023] Take by weighing 200g (fine powder of 40 order granularity) of the vine powder that pulverizes after drying at 30 DEG C, choose dehydrated alcohol: deionized water=7: 3 mixed solvent 2000ml, heat and reflux under 90 DEG C and extract three times, each 2 Hours, the solvent was recovered by vacuum concentration at 45° C. to obtain 80.9 g of paste, which was dissolved in deionized water, extracted 6 times with equal volume of ethyl acetate, and concentrated to obtain 10.8 g of extract. Take 1 g of the substance, use deionized water-ethanol as the eluent, and perform column chromatography on polyamide (40 mesh) to obtain effective components, concentrate, and freeze-dry at -40°C to obtain 71.5 mg.

[0024] In vitro, 0.016 mg / ml of this component was stained with sulforhodamine B protein, and the inhibitory rate to human liver cancer cell line BEL-7402 was 63.1%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a process for preparing Gnetopsida extract having suppressing effect to human hepatic carcinoma cell line, which comprises, airing vines of Gnetopsida, drying, disintegrating into 40 mesh particles, mixing disintegrated Gnetopsida substance with 10 times by weight of solvent containing ethanol and deionized water by the volume ratio of 7:3, heating for backflow extraction, vacuum concentrating, concentrating the mixed solvent extract into extract, dissolving grease form substance with deionized water, extracting with acetic acid ethyl ester of equal volume, vacuum concentrating the vinyl acetate extract, using polyamide as fixed phase, deionized water-ethanol as flow phase for gradient elution, vacuum concentrating the eluent, freeze drying, obtaining powdered deep brown substance, finally preparing suspension with RPMI1640 culture liquid of cow's serum.

Description

technical field [0001] The present invention relates to a method in the technical field of preparation of traditional Chinese medicines, in particular to a method for preparing the extract of Mama Teng that has an inhibitory effect on human liver cancer cell lines. Background technique [0002] Maima vine is mainly distributed in southern Yunnan, Guangdong and other provinces and Southeast Asia. It is mixed with Xiaoyemai vine among the people to treat rheumatoid arthritis, old and damaged psoas muscles, sore muscles and bones, bruises, ulcer bleeding, bronchitis, etc. disease. Pharmacological animal experiments have confirmed that the ethanol extract of Matachibana vine has obvious anti-inflammatory effects on rat egg white and yeast arthritis, but there is no literature report on anti-tumor. [0003] Through the retrieval of prior art documents, Chen Hao and Lin Mao mentioned in the 8th issue of "Chinese Herbal Medicine" in 1999 "Research on the Chemical Components of Day...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K36/00A61P35/00
Inventor 代光辉姚柳利张永煜
Owner SHANGHAI JIAO TONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com