Method for culturing Clitocybe maxima from cattail scraps
A technology for cattail cuttings and big cup mushrooms is applied in the field of preparation of special culture materials, which can solve the problems of wasting resources and not being fully and effectively developed and utilized, and achieves the effects of low production cost, reduced cultivation cost, and reduced damage.
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[0037] Embodiment: utilize cattail to cultivate the method for cup mushroom, and the cup mushroom strain is purchased in the market.
[0038] Step A. Preparation of Liquid Strains
[0039] First-class liquid culture medium formula: 20% potato, 3% bran, 2% glucose, KH 2 PO 4 0.3%, MgSO 4 0.15%, VB 1 10mg / L, CMC 0.3%, natural pH;
[0040] Secondary liquid culture medium formula: cattail 5%, potato 20%, bran 3%, glucose 2%, KH 2 PO 4 0.3%, MgSO 4 0.15%, VB 1 10mg / L, CMC 0.3%, natural pH; among them, cattail powder is crushed and sieved through an 80-mesh sieve to obtain a powder, boiled with potatoes and bran for 30 minutes, filtered to remove slag to obtain an extract.
[0041] Use a 500mL Erlenmeyer flask with a liquid volume of 200mL for the preparation of the first-level liquid strains. After autoclaving at 121°C for 30 minutes, insert commercially available PDA slant strains under aseptic conditions, and the inoculation volume of each bottle is 4 pieces of 0.5cm ×0....
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