Method for preparing clitocybe maxima liquid strains by using yellow serofluid as main raw material

Abstract

The invention relates to a method for preparing clitocybe maxima liquid strains by using yellow serofluid as a main raw material. The method comprises the follow steps: A, pretreatment of yellow serofluid: filtering and sub-packaging fresh yellow serofluid, then conducting pasteurization for 30 minutes at 62 DEG C, and reserving the sterilized yellow serofluid for later use; B, by using the yellow serofluid as the raw material and using waste molasses as a substrate, selecting and determining a liquid fermentation medium including 0.5-3.0 percent of waste molasses, 0.1 percent of MgSO4.7H2O, 0.2 percent of KH2PO4, 0.01g / L of VB1 (Vitamin B1), 0.05-1.5 percent of thickening agent, 0.2 percent of weed tree sawdust powder and the balance of pretreated yellow serofluid; regulating pH to 4.5-6.5; C, ensuring the liquid fermentation conditions as follows: pH is 4.5-6.5, the inoculum size is 10 percent, the temperature is 25-31 DEG C, the rotating speed of shaking table is 160r / min, and cultivating time is 49-120 hours; and D, inoculating peach oyster-cap fungus liquid strains obtained in the step C through fermentation into a barley seed substrate strain bottle with weed tree sawdust powder according to 10% of the inoculum size. The method fully utilizes nutrient contents of yellow serofluid and waste molasses and is beneficial to shortening the strain preparation cycle and production cycle of clitocybe maxima.

Description

technical field [0001] The invention relates to a method for preparing a cup mushroom liquid strain by using yellow pulp water as a main raw material, and belongs to the technical field of edible fungus liquid fermentation. Background technique [0002] my country is the hometown of soybeans, with a history of thousands of years of soybean planting and consumption. There are many types of soybean products and their consumption is large. Yellow pulp water is the wastewater generated during the production of soybean products. During the processing of tofu, add water about 10 times the dry weight of soybeans to grind it into pulp, and about 1 / 2-1 / 3 of the liquid will be used in the subsequent processing. Because of its yellow color, it is also called "yellow swill". Yellow pulp water is rich in nutrients, including a large amount of protein, sugar, oil and a small amount of physiologically active ingredients, and contains a variety of metal elements such as calcium, iron, zinc...

AI Technical Summary

Problems solved by technology

[0004] Yellow pulp water and waste molasses are two by-products of food production that are rich in nutrients. The former is rich in protein, and the latter is rich in carbohydrates. The combination of the two can be used as a good liquid medium for edible fungi. Fermentation research, the study of obtaining polysaccharides by fermenting edible fungi with yellow pulp water as a culture medium has been reported, but there is no report on the preparation of liquid strains from yellow pulp water and waste molasses fermenting cup mushrooms

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