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Clitocybe clavipes fungus extract as well as preparation method and application thereof

A technology of fungal extract and stick-handled cup, which is applied in the field of stick-handled cup umbrella fungus extract and its preparation, which can solve the problems of short retention time, exhaustion, confusion, etc.

Pending Publication Date: 2020-08-11
新疆维吾尔自治区中药民族药研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Clitocybe cl (Clitocybe cl Clitocybe fungus extract and its preparation method and application vipes (Pers.) Fr.) is a higher fungus of the genus Clitocybe, which grows scattered or in clusters in the forest in summer and autumn , in the fourth national resource census, during the field investigation and visits, it was found that the stick handle cup umbrella is very popular among the local people as an edible mushroom. According to folklore, this mushroom seems to be particularly effective in the treatment of liver cancer and gastric cancer. The general application principles can be used for Treatment of acute stomach pain, anti-allergic, but heavy drinking within 1 to 2 days after eating this mushroom can cause severe headache, dizziness, confusion and exhaustion
As a kind of higher fungus, Umbrella spp. grows only when the environment is suitable and does not persist for a long time. So far, there are no research reports on the chemical composition and pharmacological activity of Umbrella spp. at home and abroad. Therefore, It is of great significance to develop and utilize the stick handle cup umbrella

Method used

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  • Clitocybe clavipes fungus extract as well as preparation method and application thereof
  • Clitocybe clavipes fungus extract as well as preparation method and application thereof
  • Clitocybe clavipes fungus extract as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: the C. clump fungus extract is obtained as follows: the first step, after mixing the required amount of rice and distilled water, obtain the rice culture medium after sterilization and cooling, wherein, rice and Distilled water is 3:4 by weight; in the second step, after growing on the PDA plate for 30 days, the C. cicadae fungus strain is divided into small pieces, inoculated into rice culture medium and cultivated for 30 days to obtain C. cicadas. Bacterial flora; the third step, at room temperature, the rice culture medium that will contain the Agaricus clavium fungus flora is soaked with ethyl acetate and ultrasonicated, and placed for 7 days, and the primary extract and the primary filter residue are obtained after filtration, and the primary The filter residue was soaked in ethyl acetate and ultrasonically placed for 7 days, and the secondary extract and the secondary filter residue were obtained after filtration. The secondary filter residue was soak...

Embodiment 2

[0025] Embodiment 2: As an optimization of the above embodiment, in the first step, the sterilization temperature is 115° C. to 120° C., and the sterilization time is 30 minutes to 35 minutes.

Embodiment 3

[0026] Embodiment 3: As an optimization of the above embodiment, in the second step, the culture temperature of the rice medium is 23°C to 27°C.

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Abstract

The invention relates to the technical field of preparation of a clitocybe clavipes fungus extract, in particular to a clitocybe clavipes fungus extract as well as a preparation method and applicationthereof. The clitocybe clavipes fungus extract is obtained through the following steps: culturing a clitocybe clavipes fungus strain on a PDA flat plate, inoculating a rice culture medium for culture, finally, performing extraction on a culture solution for three times with ethyl acetate, mixing extraction solutions, and carrying out pressure reduction and concentration to obtain the clitocybe clavipes fungus extract. The invention discloses that the clitocybe clavipes fungus extract has better inhibition effects on SGC-7901 and Hela cells for the first time, so that the clitocybe clavipes fungus extract can be applied to preparation of tumor preventing drugs, antitumor drugs and tumor preventing healthcare products.

Description

technical field [0001] The invention relates to the technical field of preparation of Umbrella clumpumba extract, and relates to a fungus extract from Umbrella lanceolata and its preparation method and application. Background technique [0002] Clitocybe cl (Clitocybe cl Clitocybe fungus extract and its preparation method and application vipes (Pers.) Fr.) is a higher fungus of the genus Clitocybe, which grows scattered or in clusters in the forest in summer and autumn , in the fourth national resource census, during the field investigation and visits, it was found that the stick handle cup umbrella is very popular among the local people as an edible mushroom. According to folklore, this mushroom seems to be particularly effective in the treatment of liver cancer and gastric cancer. The general application principles can be used for Treatment of acute stomach pain, anti-allergic, but heavy drinking within 1 to 2 days after eating this mushroom can lead to severe headache, di...

Claims

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Application Information

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IPC IPC(8): C12N1/14A23L31/00A23L33/10A61K36/07A61P35/00C12R1/645
CPCA23L31/00A23L33/10A23V2002/00A61K36/07A61P35/00C12N1/14A23V2200/308A23V2250/208A23V2300/14A23V2300/48
Inventor 石磊岭马国需魏鸿雁金悦仙马晓玲樊从照关永强宋海龙陈刚郭雄飞
Owner 新疆维吾尔自治区中药民族药研究所
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