Method for preparing edible fungus protein extract with antitumor efficacy

A protein extract, edible fungus technology, applied in medical preparations containing active ingredients, food preparation, anti-tumor drugs, etc., can solve problems such as many side effects, leukopenia, and reduced immunity.

Active Publication Date: 2013-09-11
承德市御今农业发展集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current cancer treatment methods mainly include surgery, chemotherapy, and radiotherapy. Chemical drugs generally act directly on the targeted organs, with quick results, but there are many side effects. The most serious side effect is bone marrow suppression. The most painful thing for patients is nausea, vomiting and other digestive problems. Road symptoms, while chemotherapy drugs can reduce the body's immunity, causing a sharp drop in white blood cells

Method used

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  • Method for preparing edible fungus protein extract with antitumor efficacy
  • Method for preparing edible fungus protein extract with antitumor efficacy
  • Method for preparing edible fungus protein extract with antitumor efficacy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] Embodiment 1, the preparation of the edible fungus protein extract with antitumor effect

[0081] 1. Preparation of 11 kinds of edible fungus fruiting bodies

[0082] The following 11 kinds of edible fungal fruiting bodies were prepared according to the following methods: Auricularia chinensis, yellow mushroom, oyster mushroom, Milopodium vermilion, Cordyceps militaris, oyster mushroom, black fungus, big cup umbrella, enoki mushroom, long root mushroom and yellow umbrella subentity.

[0083] 1.1 Prepare 10 kinds of edible fungus fruiting bodies

[0084] Inoculate 10 kinds of edible fungus slant strains, namely, Auricularia auricula, Ulmus chrysanthemum, Abalone mushroom, Vermilion microporus, Pleurotus eryngii, black fungus, big cup umbrella, Flammulina velutipes, Long root mushroom and yellow umbrella, into the first-level culture medium Activated in 25°C constant temperature culture, inoculate it into the secondary seed medium after the mycelium is full of the test ...

Embodiment 2

[0105] Example 2: The protein extract of edible fungus with anti-tumor effect inhibits the proliferation of tumor cells

[0106] 1.1 Tested cell lines

[0107] Human liver cancer HepG2 cells (purchased from ATCC, USA), human breast cancer MCF-7 cells (purchased from ATCC, USA) and human lung adenocarcinoma A-549 cells (purchased from ATCC, USA).

[0108] 1.2 Experimental method

[0109] 1.2.1 Cell lines and cell culture

[0110] Human liver cancer HepG2 cells, human breast cancer MCF-7 cells and human lung adenocarcinoma A-549 cells were activated and passaged according to conventional culture methods. Among them, the subculture and activation medium of human liver cancer HepG2 cells is DMEM-high glucose + 1% double antibody + 10% FBS (in DMEM-high glucose (Hyclone, SH30022.01B) a mixture of penicillin and streptomycin (penicillin 10000U / mL, streptomycin 10000μg / mL) and fetal bovine serum (FBS, Hyclone, SV30087.02), so that the final volume percentage of the former two is 1...

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Abstract

The invention discloses a method for preparing an edible fungus protein extract with antitumor efficacy. The preparation method comprises the following steps of: 1) extracting by water after crushing edible fungus sporocarp, collecting a water-soluble matter to obtain an edible fungus water-soluble extract; 2) carrying out ammonium sulfate precipitation of which the saturation level is 80% on the edible fungus water-soluble extract, collecting sediments, and obtaining the edible fungus protein extract with the antitumor efficacy after dialyzing the sediments by deionized water, wherein the edible fungus is at least one of the following funguses: auricularia polytricha, pleurotus citrinopileatus, abalone mushroom, pycnoporus cin, cordyceps militaris, pleurotus djamor, black fungus, clitocybe maxima, flammulina velutipes, oudemansiella radicata and pholiota adipose. The edible fungus protein extract disclosed by the invention has a significant proliferation inhibition role on an HepG2 cell, an MCF-7 cell and an A-549 cell, and can be used for preparing a product for resisting tumors or tumor cells.

Description

technical field [0001] The invention relates to a preparation method of edible fungus protein extract with antitumor effect. Background technique [0002] At present, according to the statistics of the World Health Organization (WHO), 10 million people in the world suffer from cancer every year, and about 60,000 people die from cancer, accounting for 12% of the global death toll. There are 1.8 million new cancer patients and 1.4 million deaths every year in my country. On average, 1.3 people die of cancer every 3 minutes, and the incidence of cancer is on the rise. Last year, the number of new cancer cases was about 1.39 million, accounting for the world's cancer 17% of the total, basically close to 1 / 5. Among them, lung cancer is one of the most common and deadly malignant tumors in the world, and its incidence rate growth rate ranks first among all malignant tumors. Liver cancer has a relatively high incidence rate in the adult population in my country, and is a common dis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/06A61K36/066A61K36/07A61K38/00A23L1/305A23L1/28A61P35/00
Inventor 赵爽许峰刘宇耿小丽王守现王兰青
Owner 承德市御今农业发展集团股份有限公司
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