Method for extracting and separating paclitaxel and taxones compounds from yew

Abstract

The invention discloses a method for extracting and separating taxol and other taxane compounds from yew. The method includes the steps of extraction, degreasing, crude separation to an organic phase and an aqueous phase respectively, refined separation with reverse-phase chromatography, and crystallization. The invention sequentially adopts the ethanol of 80 to 95 percent, 60 to 75 percent and 20 to 55 percent to carry out gradient extraction to the yew material and also to fully extract taxane compounds with different polarities so as to lead the total extraction rate of the taxane compounds to be raised to more than 90 percent from 70 percent of the traditional art; the simultaneous extracting of 8 different tazane compound monomers from one set of the yew material is realized by the invention and the purity of each monomer can reach more than 98 percent; an aqueous phase substance which is generated in the extraction process abandoned by the traditional art is fully utilized to raise the utilization rate of the taxane compounds in the yew by 10 to 20 times, therefore, the bottleneck problem of industrial production of the lack of yew material is effectively solved.

Description

(1) Technical field: [0001] The invention relates to a method for extracting and separating paclitaxel and other taxane compounds from natural plants, in particular to a method for extracting and separating paclitaxel and other taxane compounds from yew. (two) background technology: [0002] Paclitaxel (trade name Taxol) is a diterpene compound isolated from Taxus genus with unique anticancer effect. It is widely used clinically in the chemotherapy of ovarian cancer, breast cancer, small cell and non-small cell lung cancer, esophageal cancer, head and neck cancer, chemotherapy-resistant leukemia and other cancers, and has definite curative effect. As the only new anticancer drug that promotes microtubule polymerization, taxol products Taxol and Taxotere have become one of the main anticancer drugs. The method for preparing paclitaxel includes: 1) extracting Taxol from yew or other renewable taxus plants; 2) semi-synthesizing Taxol or Taxotere from other natural taxane (10-d...

AI Technical Summary

Problems solved by technology

So far, only the extraction and semi-synthesis methods have achieved industrialization. Due to the complex structure of paclitaxel, the reported total synthesis methods have long routes, low yields, and high costs, and it is difficult to meet the requirements of industrialization.