Method for recycling albumin in Cohn's fraction IV precipitate

A technology of albumin and pH value, which is applied to the preparation method of albumin peptide, serum albumin, and peptide, and can solve the problem of low recovery rate

Active Publication Date: 2012-06-13
广东卫伦生物制药有限公司
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  • Summary
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Problems solved by technology

[0004] The hot ethanol method is to prepare the protein liquid by precipitation of component IV, heat to denature and solidify the miscellaneous protein in the protein liquid, and then separate the solid and liquid through pressure filtration, and concentrate the filtrate by ultrafiltration to obtain crude albumin, such as CN1660898A, CN1660903A public method, but the recovery rate is not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0014] 1. Thawing and dissolving of component IV precipitate

[0015] Take out the component IV precipitate from the cold storage below -30°C, weigh it and place it in a clean stainless steel reaction tank, pump into the reaction tank a 0-2°C, 0.75wt% NaCl solution that is three times the weight of component IV , stirred to dissolve, and the reaction solution became a paste.

[0016] After complete dissolution, the pH value of the reaction solution was adjusted to 6.90±0.10, and stirred for 5-7 hours under the pH condition, and the stirring speed was 1400-1500 rpm.

[0017] Add 0-2°C, 0.75 wt% NaCl solution 5 times the weight of component IV, and stir well.

[0018] 2. Precipitation and separation of other proteins

[0019] Spray and add acetic acid / sodium acetate buffer solution with a pH value of 4.0 to the reaction solution at a rate of 150-200ml / min, adjust the pH value of the reaction solution to 5.70±0.05, and lower the temperature of the reaction solution to -0.5±0.5°...

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Abstract

The invention discloses a method for recycling albumin in Cohn's fraction IV precipitate. By adopting the method, albumin can be fully separated from other proteins in fraction IV through selecting pH value, alcohol concentration, acidity and alkalinity and temperature, 110-120g of albumin can e prepared from per kilogram of fraction IV, and the total extraction rate of albumin in blood plasma isimproved to over 93 percent.

Description

technical field [0001] The invention relates to a method for separating blood products, in particular to a method for separating and recovering albumin from Cohn's component IV precipitation by adopting a low-temperature ethanol method. Background technique [0002] The low-temperature ethanol process for separating plasma proteins has a history of 60 years since it was established in the 1940s. The Cohn 6 method created by Professor Cohn and the development team led by him has become a classic method for albumin separation and is the basis of the internationally accepted plasma albumin separation process. The various albumin separation methods used subsequently are still based on the five parameters of pH value, temperature, ethanol concentration, ionic strength and protein concentration in the Cohn 6 method. Through different parameter combinations, other protein products can be separated. separate. [0003] A small amount of albumin remained in the Cohn's Fraction IV pe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/765C07K1/14
Inventor 黄锦程汪伟军王文杰黄邦春黄育升黄进义陈成坤黄国华
Owner 广东卫伦生物制药有限公司
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