A method for extracting effective ingredient from stem of Schisandra chinensis vine by enzyme assisted extraction

A Schisandra vine, enzyme-assisted technology, which is applied in the directions of medical preparations containing active ingredients, plant/algae/fungus/moss ingredients, pharmaceutical formulations, etc., can solve the problems of poor biological activity, low bioavailability, high price and the like, Achieve the effect of low cost, high efficiency and fast speed

Inactive Publication Date: 2019-01-25
JILIN AGRICULTURAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After research, it is found that enzyme assistance in the extraction of Schisandra is mostly limited to polysaccharides and other substances, and the enzymes used are mostly cellulase, helicase, mannanase or a variety of compound enzymes, which have certain limitations, not only the price Expensive, low bioavailability, and poor biological activity (see: Zhang Nannan, et al. Star point design-response surface method to optimize the enzymatic extraction process of Schisandra chinensis polysaccharides, China Pharmacy, 2016; Song Haiyan, et al. Response surface optimization enzymatic extraction of Schisandra chinensis Technology and antioxidant activity of polysaccharides, Food Industry, 2016)

Method used

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  • A method for extracting effective ingredient from stem of Schisandra chinensis vine by enzyme assisted extraction
  • A method for extracting effective ingredient from stem of Schisandra chinensis vine by enzyme assisted extraction
  • A method for extracting effective ingredient from stem of Schisandra chinensis vine by enzyme assisted extraction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1. Screening of enzymes in enzyme-assisted extraction of active ingredients

[0022] Take the dried schisandra cane, crush it, pass through a 40-mesh sieve, mix a certain amount of acetic acid aqueous solution with pH = 4.5 and a certain amount of enzyme, and add it into it, and add the fruit at 0%, 4%, 5%, 6%, and 7% respectively. Gluease, cellulase, laccase, solid-liquid ratio 1:30, incubate at 40°C for 5h, 60 mL absolute ethanol ultrasonic extraction twice, 30 min each time, combine supernatants, concentrate to dryness under reduced pressure, apply High-performance liquid chromatography was used to detect the content of active ingredients in the cane extract of Schisandra chinensis, and lignans were taken as an example to compare the effects of different enzymes on the extraction rate, such as figure 1 ; Compare according to three kinds of enzyme activity and application conditions, as table 1:

[0023] Table 1. Comparison of storage conditions and applicat...

Embodiment 2

[0026] Example 2. Optimizing enzyme-assisted extraction of active ingredients in Schisandra vine stems

[0027] In addition, there are many factors that affect the extraction rate of the active components of Schisandra chinensis cane, such as the ratio of solid to liquid, enzymatic hydrolysis temperature, enzymatic hydrolysis time, enzyme dosage and pH and other factors. According to the research and combined with the preliminary experiment, three factors that have a greater impact on the extraction rate were finally selected, namely the amount of enzyme (0%, 6%, 7%, 8%, 9%, 10%), enzymatic hydrolysis temperature (30°C , 35°C, 40°C, 45°C, 50°C) and enzymatic hydrolysis time (0h, 2h, 3h, 4h, 5h, 6h) for single factor experiments. attached figure 2 : (Taking the extraction rate of lignans as an example).

[0028] According to the above data of Example 1 and Example 2, the degree of influence of the three factors on the extraction rate and the practical conditions of the three...

Embodiment 3

[0029] Embodiment 3: Comparison of different extraction methods of schisandra cane

[0030] Preparation method 3.1: Take the dried Schisandra cane, crush it, pass through a 40-mesh sieve, the ratio of solid to liquid is 1:30 (g / mL), without adding enzymes, incubate at 40°C for 3 hours, ultrasonically extract with absolute ethanol for 30 minutes, extract 3 times, and combine The extract was concentrated under reduced pressure to obtain the schisandra cane extract.

[0031] Preparation method 3.2: take the dried schisandra cane, crush it, pass through a 40-mesh sieve, add 8% laccase, the ratio of solid to liquid is 1:30 (g / mL), incubate at 40°C for 3 hours, extract with absolute ethanol for 30 minutes, and extract 3 times , combined extracts, concentrated under reduced pressure to obtain Schisandra cane extract.

[0032] Preparation method 3.3: Take the dried Schisandra cane, crush it, pass through a 40-mesh sieve, add 8% laccase, the ratio of solid to liquid is 1:30 (g / mL), incu...

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Abstract

The invention belongs to the technical field of industry and mainly discloses an enzyme-assisted application in extracting lignans and triterpenoids from the stem of Schisandra chinensis. The experimental results show that: A laccase-assisted extraction method for effective component from stem of Schisandra chinensis vine in comparison with enzyme-free extraction, the extraction rate of lignans and triterpenoidssuch as schizandronic acid was 2.95% and 2.78% respectively. The laccase-assisted extraction of active ingredients from the stem of Schisandra chinensis can give full play to the advantages of enzyme biotechnology. This method is low in cost, high in speed and high in efficiency and is suitable for large-scale industrialized production.

Description

technical field [0001] The invention belongs to the technical field of extracting active ingredients of medicines in medicine, mainly relates to a method for enzyme-assisted extraction of active ingredients in schisandra canes, and significantly improves the application of lignans and triterpenoids in industrial production. Background technique [0002] Schisandra chinensis Schisandra chinensis (Turcz.) Dry ripe fruit of Baill. It was first recorded in "Shen Nong's Materia Medica" and listed as top grade. It is a variety recorded in the previous editions of "Chinese Pharmacopoeia", and it is one of the important commonly used medicinal materials in my country. It is an item "both food and medicine" announced by the Ministry of Health, and is often used as herbal medicine and food additives. Pharmacological studies have shown that lignans in Schisandra chinensis have liver and kidney protection, anti-tumor, and anti-diabetic effects, and triterpenoids have liver protection,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/79A61K36/57A61K125/00
CPCA61K36/57A61K36/79A61K2236/33A61K2236/39A61K2236/51
Inventor 李伟李艳姿张伟哲刘伟王梓任珅汤立民
Owner JILIN AGRICULTURAL UNIV
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