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Method for producing silage using compound microorganism fermentation straws

A technology of compound microorganisms and silage feed, applied in the field of production of silage feed, to achieve the effects of promoting rapid growth, facilitating accumulation, and facilitating large-scale use

Inactive Publication Date: 2012-07-18
青海鲁青饲料科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In these aspects, scholars at home and abroad have done a lot of research work, but for comprehensive solutions to inhibit spoilage bacteria in the feed fermentation process, prevent secondary fermentation, effectively control organic acids and degrade lignocellulose, reduce costs, and be easy to use, etc. there are still problems

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: the preparation method of mixed starter

[0039] 1) The preparation process of Aspergillus niger is:

[0040] A. Incline cultivation method

[0041] Test tubes were sterilized at 121°C for 20 minutes, placed on an inclined plane, cooled, and inoculated. Cultivate at 30°C until black spores cover the slope.

[0042] B.K culture bottle spores

[0043] Take 10°Brix wort and add 2% agar, put it into a 500mL K-type culture bottle, sterilize it at 121°C for 20min, spread it on an inclined surface and cool it down. Insert 1mL of spore suspension to ensure that the suspension is inoculated on the entire surface of the culture medium; put it sideways into an incubator, and cultivate at 30°C until the black spores cover the slope.

[0044] C. Solid-state scale-up culture

[0045] Make K-type bottle spores into spore suspension, and the spore concentration is greater than 1.0×10 8 individual / mL. Take 200kg of solid medium (140kg of bran, 60L of 10°Brix wort), ...

Embodiment 2

[0051] 200g of Aspergillus niger spores obtained by fermentation, 100g of Lactobacillus rhamnosus and 100g of Lactobacillus plantarum were mixed to form a composite microbial starter. Prepare silage feed in the following way with above-mentioned composite microbial fermentation agent:

[0052] 1) Raw material preparation: select 1 ton of non-rotten rice straw, corn stalks, and leguminous stalks, respectively, and cut them to a length of 2 to 5 cm.

[0053] 2) Strain activation: Pour 500 g of the above-prepared mixed microbial bacterial agent into 100 liters of 1°Brix wort, stir well and mix evenly, and activate it after 2 hours at 35°C.

[0054] 3) Inoculation loading: Raw materials are piled up in layers, and when the pile is 20cm thick, spray the bacteria solution once. The total pile height should not exceed 2 meters.

[0055] 4) Aerobic culture: place in the air for 10 days in summer.

[0056] 5) Anaerobic culture: seal with a plastic film, and it can be eaten by livest...

Embodiment 3

[0060] Mix 100g of fermented Aspergillus niger spores, 100g of Lactobacillus rhamnosus and 100g of Lactobacillus plantarum, the ratio of Lactobacillus rhamnosus is 30.0*10 9 per gram, the number of Lactobacillus plantarum is 5*10 9 pc / g, the number of Aspergillus niger spores is 20*10 8 pcs / g.

[0061] Prepare silage feed in the following way with the above-mentioned mixed bacteria starter:

[0062] 1) Raw material preparation: Select 1 ton of non-rotten rice straw and cut it to a length of 2 to 5 cm.

[0063] 2) Strain activation: Pour 700g of the above-prepared mixed microbial bacterial agent into 100 liters of 2°Brix wort, stir and mix well, and activate it after 3 hours at 25°C.

[0064] 3) Inoculation loading: Raw materials are piled up in layers, and when the pile is 30cm thick, spray the bacteria solution once. The total pile height should not exceed 2 meters.

[0065] 4) Aerobic culture: place in the air for 5 days in summer.

[0066] 5) Anaerobic culture: seal w...

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Abstract

The invention discloses a method for producing silage using compound microorganism fermentation straws. The method includes that a leavening agent is added into a silage raw material, microorganisms grow rapidly under the aerobic and anaerobic conditions, a large quantity of cellulase is secreted to decompose cellulose, protein is decomposed by protease to generate free amino acids, and simultaneously a large quantity of metabolic products of lactic acids and the like are generated. Potential of hydrogen (pH) value of the processed silage is decreased, and sundry fungi are basically absent, thereby the silage is high in nutritional value, easy to digest and applicable to livestock.

Description

technical field [0001] The invention belongs to the bacteria and its mixed bacteria starter in the field of microbial feed additives, and also relates to a method for producing green storage feed by using the mixed bacteria starter. Background technique: [0002] my country is rich in straw resources, with an annual output of 579 million tons. Among them, the straw output of the three major crops of corn, wheat, and rice straw reached 439 million tons, accounting for 75.88% of the total straw output. At present, with the rapid development of animal husbandry, the dependence of animal husbandry on grain has been intensified, but the amount of straw used as feed is small, and even if it is used as feed, the utilization rate of straw and the rate of feed return are low due to improper processing and utilization. Crop straw has low nutritional value, poor palatability and low digestibility. If it is properly processed and adjusted, it can be used to feed livestock. According ...

Claims

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Application Information

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IPC IPC(8): A23K3/03
Inventor 李政王玉苏俊李绩
Owner 青海鲁青饲料科技有限公司
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