Multiple PCR (polymerase chain reaction) method for identifying salmonella enteritidis, salmonella typhimurium, salmonella pullorum and salmonella gallinarum

A technology of Salmonella enteritidis and Salmonella, which is applied in the field of multiple PCR detection of different serotypes of Salmonella, can solve the problems of fast base mutation and unsuitable bacterial identification, and shorten the diagnosis time
CN103131784AInactive Publication Date: 2013-06-05YANGZHOU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
YANGZHOU UNIV
Publication Date
2013-06-05
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a multiple PCR (polymerase chain reaction) method for identifying salmonella enteritidis, salmonella typhimurium, salmonella pullorum and salmonella gallinarum. The multi-PCR method comprises the following steps of: with the extracted DNA (deoxyribonucleic acid) of a bacterium to be detected as a template, carrying out mPCR (multiple polymerase chain reaction) amplification on five pairs of primers as shown in SEQ ID NO.1-10; and carrying out agarose gel electrophoresis on mPCR amplification products, and then determining according to electrophoresis results. According to the multiple PCR method, the synthesized primers are used for carrying out PCR amplification, a diseased material can be directly ground and amplified by adopting the established multiple PCR, different salmonella serotypes can be detected in one system, serum glass plate agglutination is completed in only about six hours compared with three days for the traditional serum glass plate agglutination after separation pure culture in clinical detection, the diagnosis time is greatly shortened, and a basis is provided for clinical diagnosis.
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Description

technical field

[0001] The invention relates to a method for detecting different serotypes of Salmonella by multiplex PCR, and the serotypes mainly involved are Salmonella enteritidis, Salmonella typhimurium, Salmonella pullorum and Salmonella gallinarum typhi. Background technique

[0002] Salmonella (Salmonella) is a general term for a group of common bacteria, some of which only cause disease in humans, some only cause disease in animals, and some are pathogenic to both humans and animals. Salmonellosis is a general term for different forms of diseases caused by various types of Salmonella to humans, domestic animals and wild animals.

[0003] Pigs, poultry, dogs, and cats infected with Salmonella can cause symptoms such as gastroenteritis, abortion, and sepsis. With the expansion of intensive animal breeding and the widespread use of antibacterial drugs in clinics and animal feeds, the drug resistance of Salmonella is becoming more and more serious, and the infection ra...

Claims

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