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Freeze dried microecologic preparation for preventing and treating gallinaceous white dysentery

A probiotic, pullorum technology, applied in freeze-dried delivery, medical preparations containing active ingredients, resistance to vector-borne diseases, etc.

Inactive Publication Date: 2004-11-17
颜世敢 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the economical and effective freeze-dried probiotics in the prevention and treatment of pullorum have not yet been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0149] Freeze-dried microecological preparations - white dysentery and freeze-drying process

[0150] 1. Materials and methods

[0151] 1.1 Materials

[0152] 1.1.1 Bacteria used for production: Lactobacillus acidophilus (ATCC4356), Enterococcus faecalis (CMCC1.130) and Bacillus licheniformis (ATCC25972), provided by the General Microbiology Center of China Committee for the Collection of Microbial Cultures. After primary and secondary activation, inoculate the liquid culture medium with 10% inoculum volume.

[0153] 1.1.2 Medium: MRS medium.

[0154] 1.1.3 Lyoprotectant sucrose, glucose, sorbitol, mannitol, glycerol, sodium glutamate, etc. are analytical reagents or biochemical reagents.

[0155] 1.1.4 Equipment:

[0156] Freeze dryer, YRD-4503B type, produced by Shanghai Yucheng Drying Equipment Co., Ltd.

[0157] The anaerobic incubator is produced by HERAEUS company in Germany.

[0158] The pH211 meter is produced by Hana Instrument Company of Italy.

[0159] Consta...

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PUM

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Abstract

A freeze-drying micro-ecological preparation for prevention and cure white diarrhea, which comprises lactobacillus acidophilus with a finite number of viable bacteria numbers, enterococcus (CMCC 1.130) and bacillus licheniformis (ATCC 250970).

Description

technical field [0001] The invention relates to a freeze-dried microecological preparation for preventing and treating pullorum. Background technique [0002] Pullorum pullorum is a vertically transmitted infectious disease that seriously harms chicks caused by Salmonella pullorum pullorum. Drug control is currently an effective measure to control pullorum, but it often leads to the emergence of drug-resistant strains, drug residues, and easy recurrence after drug withdrawal. Therefore, it is the general trend to explore a more effective and environmentally friendly control measure and control agent. [0003] The growth of lactic acid bacteria requires high nutritional conditions, and most of them are strictly anaerobic. Therefore, it is difficult to cultivate under normal conditions, has poor resistance to the outside world, and is difficult to prepare and store for a long time. Wright pointed out that Lactobacillus bulgaricus is sensitive to low temperature treatment and ...

Claims

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Application Information

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IPC IPC(8): A61K9/19A61K35/742A61K35/747A61P31/04
CPCY02A50/30
Inventor 颜世敢朱丽萍任素芳曹洪敬徐培莲
Owner 颜世敢
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