Method for fermenting and decomposing gossypol in cottonseed meal by composite strains
A compound strain, fermentation and decomposition technology, applied in the application, animal feed molding or processing, animal feed and other directions, can solve the problems of high gossypol content, poor applicability, inability to feed application, etc., to improve crude protein content, improve The effect of digesting utilization and increasing product cost performance
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preparation example Construction
[0024] (1) Preparation of cotton meal raw material
[0025] Step 1. Take cotton meal, corn steep liquor, molasses, ammonium sulfate, urea and 10% aqueous sodium hydroxide solution as raw material components, grind the cotton meal with particle size ≤ 2.5mm, and mix cotton meal, corn steep liquor, Molasses, ammonium sulfate, urea and 10% sodium hydroxide aqueous solution are uniformly mixed, and the weight percentages of each component are: cotton meal 70-80%, corn steep liquor 5-20%, molasses 4.5-10%, ammonium sulfate 2 -2.5%, urea 1.5-2%, sodium hydroxide aqueous solution 1-1.5%, the raw material of cotton meal is prepared, and it is used for later use;
[0026] (2), preparation of compound strain fermentation broth
[0027] Step 1, carrying out aerobic fermentation culture of Corynebacterium, Candida utilis and Bacillus subtilis respectively, and carrying out anaerobic fermentation culture of lactic acid bacteria;
[0028] Step 2: Carry out expanded culture respectively to...
Embodiment 1
[0043] The method of decomposing gossypol in cottonseed meal by fermenting and decomposing compound strains with compound strains involves mixing the fermented liquid of compound strains with the raw material of cottonseed meal for fermentation, then drying, crushing, and packing in sealed bags to complete the decomposing of gossypol in cottonseed meal Decomposition, the method steps are as follows:
[0044] (1) Cotton meal raw material equipment
[0045] Step 1. Take cottonseed meal, corn steep liquor, molasses, ammonium sulfate, urea and 10% aqueous sodium hydroxide solution as raw material components, pulverize the cottonseed meal with a particle size of 2.5mm, and mix cottonseed meal, corn steep liquor, molasses , ammonium sulfate, urea, and 10% sodium hydroxide aqueous solution are uniformly mixed, and the percentages of the additions of each component are: the additions and percentages of each component are: 80% cottonseed meal, 5% corn steep liquor, molasses 10%, ammon...
Embodiment 2
[0059] The method of decomposing gossypol in cottonseed meal by fermenting and decomposing compound strains with compound strains involves mixing the fermented liquid of compound strains with the raw material of cottonseed meal for fermentation, then drying, crushing, and packing in sealed bags to complete the decomposing of gossypol in cottonseed meal Decomposition, the method steps are as follows:
[0060] (1) Cotton meal raw material equipment
[0061] Step 1. Take cotton meal, corn steep liquor, molasses, ammonium sulfate, urea and 10% aqueous sodium hydroxide solution as raw material components, and pulverize the cotton meal with a particle size of 2.0mm. , ammonium sulfate, urea, and 10% sodium hydroxide aqueous solution are uniformly mixed, and the percentages of the additions of each component are: the additions and percentages of each component are: 70% cottonseed meal, 20% corn steep liquor, molasses 4.5%, 2.5% ammonium sulfate, 1.5% urea, 1.5% aqueous sodium hydrox...
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