Rapid positioning method of cotton single locus quality gene in chromosome

A positioning method and chromosome technology, applied in the biological field, can solve problems such as heavy workload, achieve the effect of reducing workload and improving positioning efficiency

Active Publication Date: 2016-09-07
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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Problems solved by technology

However, using the land-sea hybrid F 2 Although it is easier to screen more polymorphic primers in the near-isogenic mixed pool constructed by the population, it is also more likely to obtain molecular markers linked to t

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  • Rapid positioning method of cotton single locus quality gene in chromosome
  • Rapid positioning method of cotton single locus quality gene in chromosome
  • Rapid positioning method of cotton single locus quality gene in chromosome

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Embodiment Construction

[0037] Generally speaking, in a successful exogenous gene transformation event, the exogenous genes introduced into the recipient are mostly integrated in a single-site manner, and the isolated progeny are inherited in a single-gene-controlled manner of quality traits. The introduction of exogenous Bt insect-resistance genes into cotton makes cotton resistant to insects. The following will take the chromosomal location of the Bt insect resistance genes of two insect resistant cotton varieties as examples to verify the effectiveness of the present invention in the location of cotton quality genes.

[0038] (1) Bt insect resistance gene localization of Guokangmian 12 (GK12)

[0039] 1.1 Test material

[0040] Taking the non-insect-resistant sea island cotton parent Shengli No. 1_ (a well-known public variety, see "Cotton Fine Varieties", page 58) as the male parent, the national resistance cotton GK12_ (a well-known public variety, see "China Cotton Varieties", 222 pages) As t...

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Abstract

The invention relates to a rapid positioning method of cotton single locus quality gene in chromosome, and belongs to the technical field of biology. The method includes establishing a near-isogenic mixed pool by using land-sea F2 positioning group, screening the gene to obtain a primer with polymorphism, detecting the DNA of 9 single strains, P-1, P-2 and F1 in a recessive gene mixed pool, recording the banding patterns of the DNA, calculating the individual number among the 9 single strains in the recessive mixed pool which has the same banding pattern with the recessive parent P-1, calculating the coincidence rate, selecting the polymorphic primer having the banding pattern coincidence rate over 60% with P-1 and having dominant or codominant F1 banding pattern, detecting the F2 segregation population by using the small amount of the primer, calculating the banding pattern, and analyzing by software to obtain the SSR primer concatenated with the quality gene. Displayed by the embodiment, the method makes the range of screening target concatenated primer by using F2 positioning group reduce over 90%, can lock the SSR primer concatenated with the target quality gene rapidly, can reduce the workload of the gene positioning effectively, and can improve the positioning efficiency of the quality gene in the chromosome greatly.

Description

1. Technical field [0001] The invention relates to a method for rapidly locating cotton quality genes in chromosomes, and belongs to the field of biotechnology. 2. Background technology [0002] The location of mass mutant genes on chromosomes is a work of finding a needle in a haystack. Among the quality gene mapping methods, the BSA (Bulked Segregation Analysis) method is currently the most widely used method, also known as the separation grouping mixed analysis method, the mixed grouping analysis method, and the group segregation analysis method. This method was first proposed by Michelmore et al. (1991), and successfully screened the markers linked to the target quality gene in lettuce. Since BSA uses a specific segregated population when building pools, and selects only the target traits when grouping, which can ensure that the genetic backgrounds of other traits are basically the same, theoretically, there should be differences between the two gene pools mainly in the...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 陈旭升周向阳赵亮狄佳春
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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