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Ramie-yield-trait-associated SSR (simple sequence repeat) markers and application thereof

A technology of marking and ramie, applied in the field of SSR marking, can solve the problem of less linkage molecular markers and so on

Active Publication Date: 2016-10-12
INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Still another object of the present invention is to provide a kind of SSR marker associated with ramie yield traits, to solve the problem that linkage molecular markers are few in ramie molecular breeding

Method used

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  • Ramie-yield-trait-associated SSR (simple sequence repeat) markers and application thereof
  • Ramie-yield-trait-associated SSR (simple sequence repeat) markers and application thereof
  • Ramie-yield-trait-associated SSR (simple sequence repeat) markers and application thereof

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Embodiment Construction

[0024] The present invention will be further described in detail below in conjunction with the accompanying drawings, so that those skilled in the art can implement it with reference to the description.

[0025] It should be understood that terms such as "having", "comprising" and "including" as used herein do not entail the presence or addition of one or more other elements or combinations thereof.

[0026] The present invention uses 107 ramie natural populations as materials, utilizes 95 pairs of SSR molecular markers with polymorphisms, analyzes genetic diversity, population structure, etc.; The traits such as identification, skin thickness, and hemp yield were repeatedly identified twice a year, and the above traits and molecular markers were associated with each other, and the SSR loci related to them were explored to map and improve QTL for ramie yield traits, etc. Research provides a scientific basis.

[0027] The invention provides an SSR marker associated with ramie ...

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Abstract

The invention discloses ramie-yield-trait-associated SSR (simple sequence repeat) markers which comprise SSR markers RAM200, RAM108 and RAM141. The invention also discloses application of the ramie-yield-trait-associated SSR markers in cultivating ramie new species. The invention also discloses application of the ramie-yield-trait-associated SSR markers in stabilizing or enhancing ramie yield. The SSR markers disclosed by the invention are associated with the ramie stem diameter, and the stem diameter is directly associated with the ramie yield. The SSR markers lay a foundation for high-quality germplasm screening, gene localization and cloning, and molecular marker assisted breeding in future. The SSR markers can be used in ramie molecular breeding and ramie yield enhancement. The SSR markers have important meanings for ramie breeding and production in China in the present stage. Meanwhile, the invention provides beneficial references for molecular marker breeding.

Description

technical field [0001] The invention relates to an SSR marker associated with ramie yield traits and its application. Background technique [0002] Ramie (Boehmerianivea L.Gaud), also known as "Chinese grass", originated in China. It is a perennial herbaceous plant of the genus Ramie in the family Urticaceae. It is a natural textile raw material with Chinese characteristics. At present, ramie is the second largest fiber crop in my country. Although the yield of ramie has been greatly improved compared with the past, increasing the fiber yield is still an important goal of ramie breeding. Crop yield traits are extremely complex quantitative traits, which are the final product of a series of growth and development processes and environmental interactions throughout the crop life cycle, and are usually controlled by multiple genes. The components of ramie fiber yield include sub-characters such as plant height, stem diameter, skin thickness, hemp yield and ramet force. Studi...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 栾明宝陈建华王晓飞许英孙志民
Owner INST OF BAST FIBER CROPS CHINESE ACADEMY OF AGRI SCI
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