Indel molecular marker closely linked with pumpkin photoperiod insensitivity and application of indel molecular marker

A molecular marker and photoperiod technology, which can be used in the determination/inspection of microorganisms, angiosperms/flowering plants, DNA/RNA fragments, etc. The effect of high contribution rate and shortened time

Active Publication Date: 2018-05-18
INST OF VEGETABLES GUANGDONG PROV ACAD OF AGRI SCI
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

However, it is difficult to select varieties that are not sensitive to photoperiod by traditional breeding methods. Generally, they need to be screened under long-term sunlight, which is time-consuming and laborious, which greatly affects the pumpkin breeding business in my country.

Method used

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  • Indel molecular marker closely linked with pumpkin photoperiod insensitivity and application of indel molecular marker
  • Indel molecular marker closely linked with pumpkin photoperiod insensitivity and application of indel molecular marker
  • Indel molecular marker closely linked with pumpkin photoperiod insensitivity and application of indel molecular marker

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Construction and Genetic Analysis of Genetic Populations

[0030] 1. Test materials Plant materials: photoperiod-insensitive and photoperiod-sensitive materials are high-generation inbred lines obtained locally in Guangdong, named PPIS and PPS respectively, see figure 1 . PPIS and PPS were crossed to obtain F1, and F1 was selfed to obtain F2, which were used for genetic analysis and mapping populations.

[0031] 2. The determination of the photoperiod insensitivity of the tested materials and the analysis of hereditary laws.

[0032] 162 individual plants of the F2 population were sown in April, and the flowering period was long-day. The flowering node of the first female flower was investigated, and Excel 2016 was used to process the data to check whether it obeyed the normal distribution.

[0033] 2. Construction of Pumpkin Genetic Map and Preliminary Positioning of Peel Color

[0034] 1. Extraction of pumpkin genomic DNA

[0035] The genomic DNA of pumpkin pa...

Embodiment 2

[0045] The method of Example 1 was used to carry out PCR amplification between the two parents, and polyacrylamide gel electrophoresis was used for detection. The performance of the parents was specific, and 33 individual plants of the F2 population were identified. The results are shown in image 3 .

[0046] image 3 It is the PCR amplification result of the indel molecular marker SEQ7593: P1 and P2 are the product band patterns of the photoperiod-insensitive female parent and the photoperiod-sensitive male parent respectively, wherein the length of the fragment amplified by P1 is 280bp; the length of the fragment amplified by P2 is 275bp . There are two types of bands in F1, there are 280bp and 275bp fragments, and there are three types of P1, P2 and F1 in the random individual plants of the F2 population.

[0047] Combined with individual plant phenotypes, it was found that the photoperiod-sensitive phenotypes were consistent with the results of PCR. The above-mentioned...

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Abstract

The invention discloses an indel molecular marker closely linked with pumpkin photoperiod insensitivity and application of the indel molecular marker, and belongs to the technical field of molecule detection. The indel molecular marker SEQ7593 is located on a tenth chromosome of a Chinese pumpkin, and is 280bp in size. The nucleotide sequence of the indel molecular marker is shown as SEQ ID NO.1.The indel molecular marker SEQ7593 can be directly used for creating an assistant breeding system of photoperiod insensitivity character molecular markers. Primer amplification designed according to the indel molecular marker can be applied to assistant breeding of pumpkin breed improvement molecules, technical support is provided for pumpkin photoperiod insensitivity molecular breeding, and timefor conventional gene positioning is shortened greatly.

Description

technical field [0001] The invention belongs to the technical field of molecular detection, and particularly relates to an indel molecular marker closely linked with the photoperiod insensitivity of pumpkin and its application. Background technique [0002] Chinese pumpkin is one of the three major cultivars of pumpkin, and its yield and planting area ranks first among the three cultivars. However, Chinese squash is a dioecious short-day plant, and most of them are sensitive to light and temperature. The suitable sowing period in southern China in spring is short (January-March), and the delay in sowing in April will reduce the differentiation of female flowers, resulting in severe yield reduction. Domestic Chinese squash The main varieties have planting geographical restrictions. For example, honeydew squash and its derivatives are not suitable for planting in the area north of the Yellow River. It can be seen that the sensitivity of light and temperature has greatly restri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13A01H6/348C12N15/1003C12Q1/6883
Inventor 钟玉娟黄河勋李俊星罗文龙谢大森吴廷全王瑞
Owner INST OF VEGETABLES GUANGDONG PROV ACAD OF AGRI SCI
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