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Mapping of eggplant fruit color epistatic gene D, and InDel molecular marker development and application thereof

A molecular marker, eggplant technology, applied in the field of molecular biology

Active Publication Date: 2017-05-24
INST OF VEGETABLES GUANGDONG PROV ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] So far, no related molecular markers and localization studies have been carried out on eggplant fruit color epistasis genes

Method used

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  • Mapping of eggplant fruit color epistatic gene D, and InDel molecular marker development and application thereof
  • Mapping of eggplant fruit color epistatic gene D, and InDel molecular marker development and application thereof
  • Mapping of eggplant fruit color epistatic gene D, and InDel molecular marker development and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Genetic population construction and genetic analysis

[0024] 1. Test materials

[0025] Plant material: 2 ginkgo-colored eggplant inbred lines bred by multiple generations of strict bagging self-breeding in Vegetable Research Institute of Guangdong Academy of Agricultural Sciences were used as male parent (Male, genotype ddPP) and female parent (Female, gene type is DDpp), and the hybrid combination F 1 , F 1 F 2 . The fruit color of the female parent material is white, and during the whole growth period, no anthocyanin synthesis can be observed in the hypocotyls, veins, flowers, and sepals; the fruit color of the male parent material is white, and the hypocotyls are slightly purple, The leaf veins are purple, the flowers are slightly purple, F 1 Hypocotyl, leaf vein, flower color and fruit color of the generation material are all purple ( figure 1 ). f 2 The segregation ratio of purple-red and ginkgo-colored plants in segregation generations was 9:7 by Chi-...

Embodiment 2

[0041] Example 2 Application of Molecular Marker InDel22522

[0042] 1. DNA preparation of F2 single plant

[0043] Two ginkgo-colored eggplant inbred lines bred by the Institute of Vegetables, Guangdong Academy of Agricultural Sciences through multiple generations of strict bagging self-breds were used as male (Male, genotype ddPP) and female (Female, genotype DDpp ), to prepare hybrid combination F 1 , F 1 F 2 , F 2 A total of 207 strains were isolated from the population. Paternal, female, F 2 The leaves of a single plant were taken at the seedling stage, DNA was extracted respectively, and the fruit color was investigated at the fruit setting stage.

[0044] 2. PCR amplification detection of molecular marker InDel22522 in male, female, and F2 segregation populations

[0045] The amplification system is 20 μL: ddH 2 O 5 μL, PCR mix (Genstar) 10 μL, 22522 F2 primer (10 μmol / L) 1.5 μL, 22522 R2 primer (10 μmol / L) 1.5 μL, DNA template (500 ng / μL) 2 μL. The PCR reactio...

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Abstract

The invention discloses a mapping result of an eggplant fruit color epistatic gene D. The gene D is mapped in a position near a nucleotide sequence Sme2.5_00538.1 on a No.10 chromosome of the eggplant. Based on the re-sequencing result of male and female parents, an InDel molecular marker closely linked with the eggplant fruit color epistatic gene D is obtained near the nucleotide sequence Sme2.5_00538.1 of the No.10 chromosome, and is named as InDel22522; the genetic distance of the marker from the epistatic gene D is 0.61 cM. The nucleotide sequence of the molecular marker InDel 22522 in the female parent has one 29-basic-group InDel more than that in the male parent. The mapping result on the eggplant fruit color epistatic gene D provided by the invention lays the foundation for the precise positioning and cloning of the eggplant fruit color epistatic gene; the InDel 22522 molecular marker and a molecular marker amplification primer can be applied to eggplant fruit color breeding practice in a way of simplicity, convenience, high speed and high flux; the eggplant fruit color property improving process is accelerated.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to the positioning of eggplant fruit color epistasis gene D and the development and application of its InDel molecular marker. Background technique [0002] Inheritance of eggplant fruit color is a complex trait controlled by multiple genes. In eggplant breeding practice, the inventors found that 2 pairs of epistasis effect genes control eggplant fruit color heredity ( figure 1 ). That is, after crossing 2 homozygous ginkgo eggplants, F 1 Substitute fruit color is purple, F 2 The segregation ratio of purple-red fruit-colored and ginkgo-colored plants was 9:7, indicating that the pericarp coloring was controlled by two epistasis gene loci, and the two ginkgo-colored eggplants were due to two independent genes controlling anthocyanin biosynthesis. And the complementary gene loci (D gene and P gene) are caused by inactivation respectively. [0003] So far, no related m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 孙保娟李植良黎振兴罗少波徐晓美
Owner INST OF VEGETABLES GUANGDONG PROV ACAD OF AGRI SCI
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