Watermelon female lines gene C1WIP1 and chromosome translocation and linkage marker

A chromosomal translocation, female line technology, applied in the field of molecular biology, can solve problems such as not yet realized, and achieve the effect of improving seed purity and reducing cost

Active Publication Date: 2014-07-23
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0006] In the current scientific research work, the design of markers based on the SNP/InDel differential sites obtained by whole genome resequencing has not yet been implemented to fine-map the watermelon

Method used

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  • Watermelon female lines gene C1WIP1 and chromosome translocation and linkage marker
  • Watermelon female lines gene C1WIP1 and chromosome translocation and linkage marker
  • Watermelon female lines gene C1WIP1 and chromosome translocation and linkage marker

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Embodiment 1

[0061] This embodiment is watermelon female line gene ClWIP1 and chromosomal translocation and linkage markers, including: the DNA sequence of watermelon female line gene gy gene ClWIP1 and its obtaining method, molecular markers linked to it and its obtaining method, and the occurrence of watermelon female line The chromosomal translocation segment of and its obtaining method.

[0062] The molecular marker linked with the watermelon female line gene gy gene has the DNA sequence described in SEQ ID NO: 1-6 in the sequence list.

[0063] The DNA sequence of the watermelon female line gene gy gene has the DNA sequence described in SEQ ID NO: 7 in the sequence listing.

[0064] The chromosomal translocation segments that lead to the appearance of watermelon female lines include: Chr.2 after chromosomal translocation 3 The translocation breakpoint position, that is, the translocation breakpoint position of Chr.2 of the watermelon female line material, has the DNA sequence describ...

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Abstract

The invention belongs to the molecular biology field, and more specifically relates to watermelon female lines gene(i) C1WIP1(/i) and chromosome translocation and linkage marker, which comprises a DNA sequence of watermelon female lines gene(i) gy(/i) gene(i) C1WIP1(/i), its acquisition method, a molecular marker linked with the DNA sequence, a chromosome translocation section due to appearance of the watermelon female lines and its acquisition method. According to the invention, a classic gene positioning method and a whole genome re-sequencing technology are organically combined, and watermelon female lines gene(i) C1WIP1(/i) is rapidly and accurately cloned; a PCR technology is used for confirming that a watermelon spontaneous mutant material XHBFGM is appeared by female lines phenotype due to chromosome translocation, and a preliminary research is carried out on the effect mechanism of (i) C1WIP1(/i); By aiming at the existence of the chromosome translocation, the molecular marker linked with watermelon female lines gene(i) C1WIP1(/i) is designed, the watermelon female lines phenotype which takes XHBFGB as a transfer material can be rapidly detected, the molecular marker is used for assistant breeding, seed production cost of watermelon is greatly reduced, and the seed purity is increased.

Description

technical field [0001] The invention belongs to the field of molecular biology, and specifically relates to watermelon female line gene ClWIP1 and chromosome translocation and linkage markers, including: the DNA sequence of watermelon female line gene gy gene ClWIP1 and its obtaining method, the molecular marker linked thereto and its obtaining method, And the chromosomal translocation segment leading to the female line of watermelon and its obtaining method. Background technique [0002] Watermelon (Citrullus lanatus) is an annual herbaceous plant belonging to Cucurbitaceae Cucumber, and is an important economic crop widely cultivated at home and abroad. my country has a history of growing watermelons since the Han Dynasty. It is one of the earliest countries in the world to cultivate watermelons, and it is also the country with the largest watermelon cultivation area and the highest yield in the world. [0003] Watermelon is a model crop for the study of plant sex differe...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12N15/29C12N15/10C12Q1/68
Inventor 许勇纪高洁张洁宫国义张海英郭绍贵任毅
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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