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Gene clwip1 and Chromosomal Translocation and Linkage Markers in Watermelon Female Lines

A chromosomal translocation, female line technology, applied in the field of molecular biology, can solve problems such as not yet realized, and achieve the effect of improving seed purity and reducing cost

Active Publication Date: 2016-04-13
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In the current scientific research work, the design of markers based on the SNP / InDel differential sites obtained by whole genome resequencing has not yet been implemented to fine-map the watermelon gy gene, and the candidate gene of the gy gene has been found in the mapped gene region, and the candidate gene has not yet been identified. Verify in other groups, and finally get the watermelon gy gene

Method used

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  • Gene clwip1 and Chromosomal Translocation and Linkage Markers in Watermelon Female Lines
  • Gene clwip1 and Chromosomal Translocation and Linkage Markers in Watermelon Female Lines
  • Gene clwip1 and Chromosomal Translocation and Linkage Markers in Watermelon Female Lines

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Embodiment 1

[0061] This example is the translocation between the female watermelon gene ClWIP1 and the chromosome and the linkage markers, including: the DNA sequence of the female watermelon gene gy gene ClWIP1 and its obtaining method, the molecular marker linked to it and its obtaining method, and the appearance of the watermelon female line Chromosome translocation segment and its obtaining method.

[0062] The molecular marker linked to the watermelon female gene gy gene has the DNA sequence described in SEQ ID NO: 1 to 6 in the sequence table.

[0063] The DNA sequence of the female watermelon gene gy gene has the DNA sequence described in SEQ ID NO: 7 in the sequence table.

[0064] The chromosomal translocation segments that cause the appearance of watermelon female lines include: Chr.2 after chromosome translocation 3 The translocation breakpoint position of Chr.2, that is, the translocation breakpoint position of Chr.2 of the watermelon female line material, has the DNA sequence descri...

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Abstract

The invention belongs to the molecular biology field, and more specifically relates to watermelon female lines gene(i) C1WIP1( / i) and chromosome translocation and linkage marker, which comprises a DNA sequence of watermelon female lines gene(i) gy( / i) gene(i) C1WIP1( / i), its acquisition method, a molecular marker linked with the DNA sequence, a chromosome translocation section due to appearance of the watermelon female lines and its acquisition method. According to the invention, a classic gene positioning method and a whole genome re-sequencing technology are organically combined, and watermelon female lines gene(i) C1WIP1( / i) is rapidly and accurately cloned; a PCR technology is used for confirming that a watermelon spontaneous mutant material XHBFGM is appeared by female lines phenotype due to chromosome translocation, and a preliminary research is carried out on the effect mechanism of (i) C1WIP1( / i); By aiming at the existence of the chromosome translocation, the molecular marker linked with watermelon female lines gene(i) C1WIP1( / i) is designed, the watermelon female lines phenotype which takes XHBFGB as a transfer material can be rapidly detected, the molecular marker is used for assistant breeding, seed production cost of watermelon is greatly reduced, and the seed purity is increased.

Description

Technical field [0001] The invention belongs to the field of molecular biology, and specifically relates to the translocation of watermelon female gene ClWIP1 and chromosome and linkage markers, including: the DNA sequence of watermelon female gene gy gene ClWIP1 and its obtaining method, molecular markers linked to it and its obtaining method, And the chromosome translocation segment that caused the appearance of watermelon female line and the method of obtaining it. Background technique [0002] Watermelon (Citrulluslanatus) is an annual trailing herbaceous plant of the Cucumber genus of the Cucurbitaceae family. It is an important economic crop widely cultivated at home and abroad. my country has a history of planting watermelon since the Han Dynasty. It is one of the earliest countries in the world to cultivate watermelon, and it is also the country with the largest watermelon cultivation area and the highest yield in the world. [0003] Watermelon is a model crop for the rese...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12N15/29C12N15/10C12Q1/68
Inventor 许勇纪高洁张洁宫国义张海英郭绍贵任毅
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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