dCAPS (derived cleaved amplified polymorphic sequence) primer pair for assisting in judging first female node and first male node of pumpkin and application of dCAPS primer pair

A primer pair, node technology, applied in recombinant DNA technology, microbial assay/inspection, biochemical equipment and methods, etc., to achieve the effect of shortening time

Active Publication Date: 2018-11-02
INST OF VEGETABLES GUANGDONG PROV ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there are no reports of dCAPS primers used to assist in judgin...

Method used

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  • dCAPS (derived cleaved amplified polymorphic sequence) primer pair for assisting in judging first female node and first male node of pumpkin and application of dCAPS primer pair
  • dCAPS (derived cleaved amplified polymorphic sequence) primer pair for assisting in judging first female node and first male node of pumpkin and application of dCAPS primer pair
  • dCAPS (derived cleaved amplified polymorphic sequence) primer pair for assisting in judging first female node and first male node of pumpkin and application of dCAPS primer pair

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1c

[0061] Example 1 cDAPS primer pair feasibility verification

[0062] The female parent P1 with low female and male nodes and the male parent P2 with high female and male nodes ( image 3 ), F1 and 30 randomly selected F2 are the plants to be tested. The first female node position and the first male node position of individual plants of P1, P2, F1 and 30 F2 populations are shown in the following table:

[0063] Table 1 The position of the first female node and the first male node of individual plants in P1, P2, F1 and 30 F2 populations

[0064]

[0065] Extract the genomic DNA of the plant to be tested using conventional methods, use primer pair 1 and primer pair 2 to carry out PCR amplification respectively,

[0066] The sequence of primer pair 1 is:

[0067] F1: 5'-CTAATCCACAGCTCATTGTGCA-3' (SEQ ID NO.5),

[0068] R1: 5'-ACGTCCCGATAACTATAGCTGGAA-3' (SEQ ID NO.6),

[0069] The sequence of primer pair 2 is:

[0070] F1: 5'-TGAACCGAGGACACCTAAGCTC-3' (SEQ ID NO.7),

[0...

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Abstract

The invention discloses an SNP molecular marker 32622 and a molecular marker 63349 which are closely linked with QTL (quantitative trait loci) of a first female node and a first male node of a pumpkinand application of the molecular markers. The located QTL have a significant incidence relation with the quantitative trait (p is smaller than 0.05), the contribution rate is relatively high, the interpretation probability of the molecular marker 32622 is 26.5%, and the interpretation probability of the molecular marker 63349 is 21.9%; and through the two molecular markers, the heights of the first female node and the first male node of the pumpkin can be respectively predicted. A dCAPS primer pair designed according to the molecular markers can be applied to the improved molecular-assisted breeding of pumpkin varieties in a simple, rapid and high-throughput manner, provides molecular-assisted selection technology support for the realization of the early identification and screening of the traits of the first female node and the first male node of the pumpkin, and greatly shortens the time of traditional gene localization; and after verification, the predicted accuracy rates of the first female node and the first male node of a plant are 77% and 83% respectively.

Description

technical field [0001] The invention belongs to the field of molecular detection, and in particular relates to a pair of dCAPS primers for assisting in judging the position of the first female node and the first male node of pumpkin and an application thereof. Background technique [0002] In the research of melon breeding, the first female node position and the first male node position are related to the early maturity of the variety, and the early maturity is the key factor affecting the market time and price of pumpkins. The key genes for flowering, and the development of molecular markers closely linked to them are of great significance for pumpkin breeding. With the deepening of scientific research and the needs of the society, the planting time is shortened. Therefore, the breeding of pumpkins with low female and male nodes is one of the important goals of pumpkin breeding in the future. Although it is feasible to breed varieties with low first female and first male n...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13
Inventor 钟玉娟黄河勋李俊星吴廷全周洋洋
Owner INST OF VEGETABLES GUANGDONG PROV ACAD OF AGRI SCI
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