63 results about "Paired design" patented technology

A system and method for the secure online storage of digital currency or crypto-currency assets, and the secure use of stored online digital currency assets for financial payment transactions and credit lending transactions in either digital currency or fiat currency. The present invention includes various methods for the encryption and secure online storage of a digital currency wallet using spliced/paired design architecture, and various methods for the integration of secure digital currency online wallets with online banking platforms, debit card devices, credit card devices, credit lending networks, merchant payment processors and credit card associations. The present invention also relates to the use of spliced/paired design architecture for non-financial applications that improve the online storage security of other types of data files and document files that are not related to digital currency or financial transactions.

A novel protective batting glove which is used specifically for protecting the most vulnerable parts of a batter's hands and wrists when the batter is standing at home plate and is gripping the end of a baseball bat and awaiting the arrival of a baseball which is thrown by the pitcher. The invention comprises a unique protective system of a design of a matched pair of batting gloves with one matched pair designed for a right handed batter and one matched pair designed for a left handed batter. For each matched pair of batting gloves, impact and shock absorbing material is incorporated onto selected portions of the exterior of the glove where the grip on the bat causes the hand to be most exposed to a pitch thrown at the batter. Alternatively, the glove has a cuff with an affixed interior impact and shock absorbing material to provide double protection for the ulna bone of an arm.

The invention discloses a liquid phase chip for detecting PIK3CA (phosphoinositide-3-kinase, catalytic, alpha) gene mutation. The liquid phase chip mainly comprises (A) a wild-type and mutant-type allele specific primer extension (ASPE) primer pair designed for mutational sites of a PIK3CA gene respectively, microspheres which are respectively coated with specific anti-tag sequences and have different colors of codes and amplification primers for respectively amplifying the target sequences of the PIK3CA gene with the corresponding mutational sites of the Exon 9 and/or Exon 20, wherein the anti-tag sequences can correspondingly complement and form pair with the selected tag sequences. The liquid phase chip has the following advantages: the coincidence rate of the detection method provided by the invention and a sequencing method is as high as 100%; the prepared liquid phase chip has very good signal to noise ratio; cross reactions do not exist between the designed probes and the anti-tag sequences; and parallel detection of various mutant types and various mutational sites on the same site can be realized.

This invention discloses a dynamic interference guiding method, and mainly solves the problem that the data rate of a user is reduced due to overlarge power expense in the interference management method. The method comprises the following steps: channel state and data information among transmitters are shared; a target communication pair and an interference communication pair design a respective precoding vector and a filtering vector; a target communication transmitter calculates the precoding vector of the interference guiding signal, so that the direction of projection component of the interference guiding signal is opposite to the direction of projection component of the interference signal in the direction of an expected signal; and then, the optimal dynamic interference guiding coefficient is calculated; the interference guiding power expense and the residual interference are compromised; hereby, the dynamic interference guiding signal is constructed; the dynamic interference guiding signal and the expected signal are sent to the target communication receiver to realize interference dynamic guiding; and at last, the receiver restores expected data. Through reasonably distributing the transmitting power of the transmitter corresponding to the interfered receiver, the spectrum efficiency of the interfered receiver is maximized, and the method is used for transmitting data when the receiver is interfered.

The fast dynamic measurement of bandwidth in a TCP network environment utilizes a single pair of packets to calculate bandwidth between two entities on a network (such as the Internet). This calculation is based upon the packet-pair technique. This bandwidth measurement is extremely quick. On its journey across a network, communication devices may delay the packet pairs. In particular, TCP networks have two algorithms designed to delay some packets with the goal of increasing the overall throughput of the network. However, these algorithms effectively delay a packet pair designed to measure bandwidth. Therefore, they distort the measurement. These algorithms are Nagle and Slow Start. The fast dynamic measurement of bandwidth implements countermeasures to overcome the delays imposed by these algorithms. Such countermeasures include disabling the application of the Nagle Algorithm; minimizing the buffering of packets by sending a “push” packet right after the packet pair; and avoiding the Slow Start Algorithm by priming it with a dummy packet.

Disclosed is an accurate tester of a superconductive-bulk magnetic-repulsion under a micro gap and a test method thereof. The tester mainly includes a double-driving mechanism, a permanent-magnetic chuck, a superconductor chuck, a liquid nitrogen cup, an optical grating displacement sensor and an S-shaped tension and pressure sensor. The tester adopts a double-driving method, a closed-loop control system and high-precision sensors to realize accurate positioning of a superconductive bulk and accurate measurement of a magnetic repulsion. The chuck structure realizes adaptive adjustment of a parallel degree of the permanent magnet-superconductor pair. The tester realizes 1mum measurement and positioning precision and 0.1 mum testing resolution so that blanks in measurement instruments of the magnetic repulsion of a superconductor bulk in the range of a micrometer gap are filled and thus testing demands for a kinematic pair/friction pair design in a micro gap in a maglev bearing can be met, and the tester has the advantages of being high in measurement precision, high in resolution and moderate in cost and the like and is worth adopting and popularizing; and the tester can also be used as a serialization product and used for teaching and scientific research of graduate students. The testing method has the advantages of being high in measurement precision, simple, convenient and practical and wide in applicable range and the like.

The invention discloses a liquid phase chip for detecting EGFR (epidermal growth factor receptor) gene mutation. The liquid phase chip mainly comprises a wild-type and mutant-type allele specific primer extension (ASPE) primer pair designed for mutational sites of an EGFR gene respectively, microspheres which are respectively coated with specific anti-tag sequences and have different colors of codes and amplification primers for respectively amplifying the target sequences with the corresponding mutational sites of the Exon 19, Exon 20 and/or Exon 21, wherein each ASPE primer comprises the tag sequences at the 5' terminal and the specific primers aiming at the mutational sites of the target gene at the 3' terminal; and the anti-tag sequences can correspondingly complement and form pair with the tag sequences selected in (A). The liquid phase chip has the following advantages: the coincidence rate of the detection method provided by the invention and a sequencing method is as high as 100%; the prepared liquid phase chip has very good signal to noise ratio; and cross reactions do not exist between the designed probes and the anti-tag sequences.

The invention discloses a specific primer pair for assisting identification of Klebsiella pneumoniae. The primer pair provided in the invention consists of DNA shown in sequence 1 of the sequence table and DNA shown in sequence 2 of the sequence table. The specific primer pair designed in the invention can be used for assisting identification about whether Bactrocera dorsalis (Hendel) carries Klebsiella pneumoniae only by means of PCR (polymerase chain reaction) and electrophoresis, and the identification is characterized by high sensitivity, simple process and no need for cloning. In order to increase accuracy, a PCR amplified product can be subjected to sequencing. The specific primer pair provided in the invention improves the efficiency of Bactrocera dorsalis (Hendel) symbiotic bacteria (Klebsiella pneumoniae), and creates conditions for further study of the coevolution relation between Bactrocera dorsalis (Hendel) and Klebsiella pneumoniae.

The invention discloses a method for designing a clutch friction pair. The clutch is a hydro-viscous driver (HVD). The method comprises the following steps of: I, acquiring rated load of the friction pair; II, selecting a friction pair material; III, designing and verifying the inner and outer diameters of the friction pair; IV, designing an oil groove of the friction pair; and V, checking the working thermal balance of the friction pair. According to the invention, the friction pair is designed and checked by fully considering the working principle and practical working condition of the HVD, so that the performance requirement of practical engineering on the HVD can be met actually. The friction pair designed with the method can be widely applied to industrial fields of HVD variable-speed energy-saving systems such as thermal power plants, water plants, chemical plants and the like. As proved by experiments, the method can fully meet the practical requirement of engineering.

The invention discloses a PCR identification method for an equine derived constituent in a traditional Chinese medicament, which comprises the following steps of : a), extracting DNA from the traditional Chinese medicament; b), performing PCR amplification on the DNA with an equine derived special primer pair designed according to a satellite sequence with equine specificity to form an amplified product; and c), performing electrophresis on the amplified product, judging whether a positive amplified strip is amplified on the DNA or not according to an electrophresis result, wherein if the positive amplified strip is amplified on the DNA, the traditional Chinese medicament is derived from an equine animal and contains equine derived material, and if the positive amplified strip is not amplified on the DNA, the traditional Chinese medicament is not derived from the equine animal and does not contain any equine derived constituents. The method of the invention is simple, sensitive and rapid in operation and low in cost, can effectively identify an equine derived product, is particularly suitable for the identification of a deeply processed horsehide gelatin which has low DNA content and very short fragment caused by deep processing, and is suitable for massive popularization and application.

The invention provides a molecular marker for detecting resistant DNA (Deoxyribose Nucleic Acid) methylation of dairy cow mastitis and a primer pair of the molecular marker. According to an amplification primer pair and a sequencing primer pair designed by the molecular marker, the resistant DNA methylation of the dairy cow mastitis can be detected. The detecting method comprises the following steps of: treating genome DNA of a to-be-detected dairy cow by bisulfate; carrying out PCR (Polymerase Chain Reaction) amplification onto a CD4 gene by utilizing the amplification primer pair; sequencing by utilizing the sequencing primer pair to match with a pyrophosphoric acid apparatus, so that the methylation degree of a CD4 gene promoter region of the to-be-detected dairy cow can be detected. The molecular marker for detecting resistant DNA methylation of dairy cow mastitis disclosed by the invention can be used for providing theoretical basis and hereditary basis for molecular breeding of the dairy cow with the mastitis.

The invention discloses a liquid chip for detecting gene point mutation. The liquid chip comprises: a primer pair designed specific to gene mutation sites, a microsphere, and a signal report molecule. In the primer pair, the last basic group at the 3' terminal of a forward primer is complementary to the basic group of a to-be-detected gene mutation site, and is not complementary to a wild-type gene. A basic group that is not complementary to mutant-type and wild-type genes is introduced into a forward primer sequence artificially. The forward primer is in connection with the microsphere through a connecting arm. A reverse primer is completely complementary to the mutant-type gene, and its 5' terminal is labeled with the signal report molecule. The invention also discloses a method for detecting gene point mutation. The method comprises the steps of: designing the primer pair specific to gene mutation sites; using the primer pair to conduct amplification on a to-be-detected mutation gene in a water-in-oil emulsion; and detecting a fluorescence signal on the microsphere surface. With the liquid chip and the method for detecting gene point mutation provided in the invention, the detection sensitivity of low abundance gene point mutation under the background of a lot of wild-type genes can be improved substantially.

The invention provides a primer, method and application based on next-generation sequencing technology to detect HBV drug-resistant mutation sites. A primer pair designed specially for a next-generation sequencing platform only needs a round of PCR, so that compared with existing detection methods based on nested PCR, the technical scheme has high stability, specificity and sensitivity. The technical scheme is especially suitable for adopting the next-generation sequencing platform to detect the HBV drug-resistant mutation sites, and especially suitable for Illumina sequencing platforms.

The invention provides a high-reliability in-series redundant type device, which comprises a single valve assembly, an inlet connector, a filter screen assembly, a throttling orifice plate and an outlet connector. The two single valve assemblies are used for controlling on-off of propellant, and the function of opening or closing the valve is achieved through axial movement of the internal movingpart. Reliable sealing when the single valve assembly is closed is achieved by pressing and attaching an internal non-metal sealing piece to the metal base. The two single valve assemblies are connected in series and fixedly welded, and the inlet connector is used for being connected with an upstream pipeline and further used for containing the filter screen assembly. The filter screen assembly isused for preventing excess materials in the working medium from entering the inner cavity of the valve; the outlet connector is used for being connected with a downstream engine. The orifice plate isused for adjusting the flow of the propellant. By means of the structural design, the kinematic pair design, the magnetic circuit design and the sealing pair design, the requirements for high sealingreliability, multiple action times, long service life, light weight and quick response can be met.

The invention discloses an SNP molecular marker 32622 and a molecular marker 63349 which are closely linked with QTL (quantitative trait loci) of a first female node and a first male node of a pumpkinand application of the molecular markers. The located QTL have a significant incidence relation with the quantitative trait (p is smaller than 0.05), the contribution rate is relatively high, the interpretation probability of the molecular marker 32622 is 26.5%, and the interpretation probability of the molecular marker 63349 is 21.9%; and through the two molecular markers, the heights of the first female node and the first male node of the pumpkin can be respectively predicted. A dCAPS primer pair designed according to the molecular markers can be applied to the improved molecular-assisted breeding of pumpkin varieties in a simple, rapid and high-throughput manner, provides molecular-assisted selection technology support for the realization of the early identification and screening of the traits of the first female node and the first male node of the pumpkin, and greatly shortens the time of traditional gene localization; and after verification, the predicted accuracy rates of the first female node and the first male node of a plant are 77% and 83% respectively.