Fluorescent labeled X-STR gene locus multiplex PCR method and application thereof

A fluorescent labeling and locus technology, applied in the field of genetic labeling, can solve the problems such as the inability to use paternity testing and personal identification of the Han population, the need for 3 to 4 weeks to arrive, and the limitation of wide application, and reach the scope of applicability of the test materials. Wide range of inspection materials and reagent consumables, high sensitivity
CN102250883AInactive Publication Date: 2011-11-23SUN YAT SEN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SUN YAT SEN UNIV
Publication Date
2011-11-23
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a fluorescent labeled X-STR locus multiplex PCR method and an application thereof; the system performs multiplex amplification analysis of 12 loci: GATA172D05, DXS6789, DXS10074, DXS10078, GATA165B12, DXS6797, DXS6803, DXS6804, GATA31E08, DXS7130, DXS9895, and DXS6810, wherein primers of the 12 loci are labeled respectively by four fluorescences of FAM, HEX, TAMRA, ROX. The method of the invention can be used to prepare a set of kit as an X-STR locus multiplex amplification kit with Chinese characteristics, and the kit is applicable to gene localization of paternity identification, individual identification, sex identification, and X-linked genetic diseases, and is especially applicable to paternity identification for sister recognition, half-blood half-sister recognition, generation-skipping recognition, etc.
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Description

technical field

[0001] The invention relates to the detection of polymorphic genetic markers in the human genome, in particular to a fluorescent label X-STR gene locus composite PCR method and its application through compound amplification of 12 STR loci. Background technique

[0002] Short tandem repeat (short tandem repeat, referred to as STR) is a type of microsatellite DNA sequence formed by tandem repetition of 2-7 base pairs as the core unit, and its fragments can be amplified by PCR technology. The genetic polymorphism of the STR locus is mainly due to the change of the number of core repeat units, and its alleles can be typed by techniques such as silver staining, fluorescent labeling and autoradiography. In the human genome, there is an STR locus every 6-10kb on average, which provides a rich source of high-information genetic markers for forensic personal identification and paternity identification.

[0003] The X chromosome STR (X chromosome short tandem repeat,...

Claims

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