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SNP (Single Nucleotide Polymorphism) molecular marker chained with pumpkin peel color gene and application thereof

A molecular marker, pumpkin skin color technology, applied in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problem of time-consuming and laborious, affecting the cause of pumpkin breeding, and achieve the effect of shortening the time.

Active Publication Date: 2017-05-10
INST OF VEGETABLES GUANGDONG PROV ACAD OF AGRI SCI
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Problems solved by technology

Although it is feasible to breed solid-color varieties by traditional breeding methods, it is time-consuming and labor-intensive, which greatly affects the pumpkin breeding business in my country.

Method used

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  • SNP (Single Nucleotide Polymorphism) molecular marker chained with pumpkin peel color gene and application thereof
  • SNP (Single Nucleotide Polymorphism) molecular marker chained with pumpkin peel color gene and application thereof
  • SNP (Single Nucleotide Polymorphism) molecular marker chained with pumpkin peel color gene and application thereof

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Experimental program
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Embodiment 1

[0035] 1. Genetic population construction and genetic analysis

[0036] 1. Plant material for the test: the dark green peel material is a high-generation inbred line isolated from Thailand, named CMO-E (P1), and the light green peel material is a high-generation inbred line obtained locally in Guangdong, named CMO-E (P1). For CMO-X (P1). F1 was obtained by crossing CMO-E and CMO-X, and F1 was self-crossed to obtain F2, which was used for genetic analysis and positioning population.

[0037] 2. Determination of the skin color of the test material and analysis of the genetic law

[0038] For 200 individual plants of the F2 population, when the fruit grows, and the color of the young fruit 2-10 days after the fruit blooms and the mature fruit 30-40 days are respectively investigated, the color of the young fruit and the color of the young fruit are investigated at the same time Among the 148 plants with mature fruit color, 107 plants are light green with spots, while 41 plants ...

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Abstract

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker chained with a pumpkin peel color gene and an application thereof. A molecular marker 63809 and a molecular marker 30774 obtained by screening are tightly chained with the pumpkin peel color gene, and the genetic distance between the two markers is 1.04cM; the SNP molecular marker can be directly applied to building of a peel color gene molecular marker assisted breeding system. A dCAPS amplification primer designed according to the two molecular markers can be used for improving molecular assisted breeding of pumpkin varieties easily, conveniently and rapidly at a high flux, technical support is provided for pumpkin appearance quality molecular breeding, and meanwhile the conventional gene mapping time is shortened greatly.

Description

technical field [0001] The invention belongs to the technical field of molecular detection, and specifically relates to a SNP molecular marker linked with a pumpkin skin color gene and an application thereof. Background technique [0002] Peel color is an important indicator of fruit appearance quality and an important factor affecting commodity value. Chinese pumpkin is one of the three cultivars of the genus Cucurbita. Foreign Chinese pumpkins are mostly yellow-skinned or dark-green-skinned varieties with solid color, while in my country, light yellow-spotted-skinned varieties are mainly cultivated, and there are fewer varieties without variegated skin. . In recent years, pure yellow or dark green leather varieties are more popular, but because most of the resources in our country are light yellow or orange varieties with piebald spots, it is still unable to meet the market's demand for pure color leather. In order to meet the needs of the consumer market, our country Som...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 钟玉娟黄河勋罗少波吴廷全李俊星
Owner INST OF VEGETABLES GUANGDONG PROV ACAD OF AGRI SCI
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