40results about How to "The test result is specific" patented technology

The invention discloses a PRV, PCV-2, PPV multiple PCR (Polymerase Chain Reaction) detection kit which comprises 40 mu L of isopyknic mixed PRV primer, PCV-2 primer and PPV primer, wherein the concentration of the PRV primer, the PCV-2 primer and the PPV primer is 10muM, buffer solution is 600 mu L, negative control is 20 mu L, PCR enzyme is 250 mu L, ultrapure water is 170 mu L, Market DL 2000 is 50 mu L, and positive control is 20 mu L. The invention aims to a condition that the PRV,the PCV-2 and the PPV exist in a same reaction system, designs three types of primers, and adopts the kit made from the three types of primers. By utilizing, the PRV,the PCV-2 and the PPV can be simultaneously detected, the specificity and sensitivity are high, the detection time is short, the detection cost is low, and a scientific basis is provided for prevention and control of pig infectious diseases.

The invention relates to a primer for detection, specifically relates to a PCR (polymerase chain reaction) detection primer for detecting enterobacter cloacae and belongs to the technical field of biological detection. The enterobacter cloacae specific PCR detection primer comprises a forward primer and a reverse primer, wherein the forward primer F is 5'-CATGACACCGGTGTTTCCCCAGT-3'; and the reverse primer R is 5'-CGGTCGGTGAAGCCCAGAACCACTA-3'. A detection method for detecting the enterobacter cloacae, disclosed by the invention, has the advantages that the required detection time is short, the specificity is strong and the sensitivity is high. The PCR detection primer disclosed by the invention can avoid the shortcomings of trivial operation, long time, low accuracy, low detection rate and the like of a traditional identification method.

The invention discloses a multiplex PCR (Polymerase Chain Reaction) detection method for salmonella typhimurium and serum variants of the salmonella typhimurium and belongs to the technical field of food safety inspection. The detection method comprises the following steps of: (1) designing amplification primers, with specific gene sequences of the salmonella typhimurium as templates; (2) extracting the DNA (Deoxyribonucleic Acid) of a sample, and carrying out amplification by using a PCR method; and (3) detecting amplification products through gel electrophoresis, and judging electrophoresis results. The invention further relates to four pairs of specific identification primers, wherein the sequences of the primers are respectively shown in SEQ ID NO: 5 and SEQ ID NO: 6, SEQ ID NO: 7 and SEQ ID NO: 7, SEQ ID NO: 9 and SEQ ID NO: 10, and SEQ ID NO: 11 and SEQ ID NO: 12. When the detection method disclosed by the invention is used to detect the salmonella typhimurium and the serum variants of the salmonella typhimurium, the detection time is short, the cost is low, the practicability is better, the detection results are specific, and the result judgment is simple.

The invention provides a detection kit of phosphorylation of sperm tyrosine. The detection kit comprises the following components: a capacitation buffer liquid, a fluorescent combined object and a fluorescent solid sealing object. The invention also provides a method for detecting the phosphorylation of the sperm tyrosine by using the kit. Compared with the prior art, according to the invention, each link of methodology is optimally designed, which includes the best buffer liquid matching of capacitation and an upstream method, a stable reaction place offering by special tissue carrier sheets, sperm double-labeling technique formulation combined with specificity, and influences to the detection result by non-sperm substances of cast-off cells in a seminal fluid are eliminated, thereby guaranteeing the methodology reliability, detection result specificity and good repeatability. Because an optimized detection scheme is adopted, the operation steps and the operation time are reduced, and the detection efficiency is improved greatly; because the optimized detection scheme is adopted, the reagent structure is simplified, and the detection cost is lowered to a greater degree; and because the operation is simple, ready-to-use available reagents are adopted, and the performance is stable.

The invention discloses a PCR (polymerase chain reaction) detection method for specificity of salmonella typhimurium. The PCR detection method includes steps: designing amplifier set sequences SEQ ID NO:2 and SEQ ID NO:3 according to a conservative and specific STM (scanning tunneling microscope) 4493 gene sequence SEQ ID:1 in a salmonella typhimurium complete genome sequence; extracting a DNA (deoxyribose nucleic acid) sample, and realizing amplification by a PCR method; and detecting an amplified product by means of gel electrophoresis and judging whether the sample contains the salmonella typhimurium or not. Particularly, if a single amplification band appears at 456bp in an electrophoresis result, the sample contains the salmonella typhimurium; and if the corresponding amplification band does not appear, the sample does not contain the salmonella typhimurium. When applied to detect the salmonella typhimurium, the PCR detection method has the advantages of short detection time, low cost, capability of detecting specificity of the detection result and simplicity in judging the result.

The invention relates to a detection method for infant salmonella PCR and nucleic acid and primer pair therein, belonging to the technical field of food safety detection; the detection method comprises the following steps: designing an amplimer according to the conserved sequence SEQ ID NO:1 in the genome DNA sequence of the infant salmonella; extracting sample DNA, amplifying by PCR method; detecting the amplified product through gel electrophoresis, and judging whether the sample contains the infant salmonella or not; and the judgment is particular as follows: if corresponding single amplification belt does not appear, the sample does not contain the infant salmonella. The invention also relates to a nucleic acid, the base sequence of which is showed by SEQ ID NO:1; the invention also relates to a pair of primer, particularly the base sequence of which is showed by the nucleic acid SEQ ID NO: 2, and SEQ ID NO: 3. When detecting the infant salmonella by adopting the invention, the detection time is short, the cost is low, the detection result is distinctive and the result is judged easily.

The invention discloses a specific primer and a method for detecting bovine derived proteus. The primer comprises a forward primer and a reverse primer. Specifically, the forward primer is F:5'-ATGCGCACACTGACCCAATTA-3', and the reverse primer is R:5'-CTGATCGCGTCCTTCAAGCC-3'. The specific primer is disclosed in SEQ ID NO.1-2. The detection method comprises the following steps of: S1: extracting thegenome DNA (deoxyribonucleic acid) of a sample to be detected; S2: using the specific primer for detecting the bovine derived proteus to carry out PCR (polymerase chain reaction) amplification on thegenome DNA of the sample to be detected; and S3: carrying out gel electrophoresis detection, carrying out photographing detection under a gel imaging system, if a DNA specific band of 306bp is in thepresence, determining that the sample contains the proteus, and otherwise, judging that the sample does not contain the proteus. The primer provided by the invention can be used for the PCR detectionof the proteus, has the characteristics of short detection time, low cost, high detection result specificity and high practicality, and a result is easy in interpretation. The detection method established by the invention utilizes a PCR technology, and therefore, the detection method has the characteristics of being convenient in operation, quick, high in sensitivity, high in specificity and thelike.

The invention relates to clostridium ghonii specific PCR detection primers and a method. Nucleotide sequence of a forward primer of the pair of clostridium ghonii specific PCR detection primers is shown as SEQ ID NO.1, and nucleotide sequence of a reverse primer is shown as SEQ ID NO.2. According to the invention, it is discovered for the first time that an encoding gene Trx of thioredoxin obeys to a highly conserved law which is different from other thioredoxin gene sequences, which keep a close genetic relationship, such as clostridium novyi, clostridium beijerinckii, clostridium sporogene and the like; and on the basis of the finding, the clostridium ghonii specific PCR detection primers are designed, so that technical difficulties in clostridium ghonii in-vivo distribution and clinical detection by virtue of a PCR technology are solved.

The invention relates to a primer pair for detecting listeria monocytogenes and a method for detecting the listeria monocytogenes. An upstream primer in the primer pair is a base sequence represented by SEQ ID NO: 2; and a downstream primer is a base sequence represented by SEQ ID NO: 3. When the method is used for detecting the listeria monocytogenes, the detection time is short and the cost is low; and a detection target has very strong specificity, specific detection result and simple result judgment.