40results about How to "The test result is specific" patented technology

The invention discloses primers and a method for detection of enterobacter cloacae O21 type, wherein the primers comprise a forward primer 5'-TGTCTTGACCGCCTAT-3' and a reverse primer of 5'-AAACCGAATGATAAGC-3'. During the detection, DNA of a sample is extracted, and the PCR amplification is performed by using the primers, and the amplified product is detected by 1% agarose gel electrophoresis. The detection method has the advantages of short required time, high specificity and high sensitivity for detecting the enterobacter cloacae O21 type, and the disadvantages of complicated operation, long time consuming, low accuracy, low detection rate and the like of a traditional identification method are avoided.

The invention relates to an immune colloidal gold test strip and a detection method for karyotype polyhedrosis viruses of bombyx mori, particularly relates to an immune colloidal gold diagnosis test strip for detecting the karyotype polyhedrosis viruses of the bombyx mori by adopting an immunochromatography technology, and a preparation method of the immune colloidal gold diagnosis test strip, and belongs to the technical field of virus epidemic disease diagnosis. The immune colloidal gold test strip comprises a colloidal gold pad marked with an antigen BmNPV-Lef4 antibody and a coated nitrocellulose membrane, wherein an upper detection line of the nitrocellulose membrane is coated by rabbit-anti BmNPV-Lef4; and a quality control line at the lower part of the nitrocellulose membrane is coated with goat-anti-mouse IgG. The immune colloidal gold test strip is used for detecting the karyotype polyhedrosis viruses of the bombyx mori; compared with a traditional colloidal gold detection method, a double-antibody sandwich method is adopted when the colloidal gold test strip is prepared and a concentration proportion of two virus antibodies is regulated, so that the specificity, the sensitivity and the stability of a detection result can be effectively guaranteed; the detection sensitivity is high and the detection method is simple and convenient and is applied to the detection of the karyotype polyhedrosis viruses of the bombyx mori for the first time.

The invention discloses primers and a method for detection of enterobacter cloacae O20 type, wherein the primers comprise one of two groups of nucleotide sequences, a forward primer of the first group of nucleotide sequences is 5'-AGAATCTTACACTGGCTTA-3', and the reverse primer is 5'-CTTAATACACCTGGCACA-3'; the forward primer of the second group of nucleotide sequences is 5'-TTTGGCTATGGCCGTATT-3', and the reverse primer is 5'-GTGCAGATGCCTGATGAA-3'. During the detection, DNA of a to-be-detected sample is extracted, and the PCR amplification is performed by using the first group of nucleotide sequences or the second group of nucleotide sequences as the primers, and the amplified product is subjected to electrophoresis detection. The method avoids the disadvantages of a traditional identification method, and has the advantages of short time, high specificity and high sensitivity for detecting the enterobacter cloacae O20 type.

The invention discloses a PCR detection method of Riemerella anatipestifer. The detection method comprises the following steps: 1, amplification primers are designed according to a conserved sequence of gyrB gene in a Riemerella anatipestifer genomic DNA sequence, wherein the conserved sequence is represented by SEQ ID NO:1; 2, DNA is extracted from a sample and is subjected to PCR amplification; 3, the obtained amplified product is detected through gel electrophoresis to determine whether the sample contains Riemerella anatipestifer; 5, if there is a corresponding single amplified strip in the electrophoresis result, the sample contains Riemerella anatipestifer; and 6, if there is no corresponding single amplified strip in the electrophoresis result, the sample contains no Riemerella anatipestifer. By adopting the detection method of the invention to detect Riemerella anatipestifer, the detection time is short, the cost is low, the detection result is specific, and the result determination is simple.

The invention relates to a multiple PCR identification method of salmonella serogroups A, B, C1, C2 or D, belonging to the technical field of food safety detection. The method comprises the following steps: step 1: respectively designing specific amplification primer pairs according to a base sequence such as 5 nucleic acids as shown in SEQ ID NO:1-5; and step 2: detecting a sample by utilizing the specific amplification primer pairs obtained in the step 1 and adopting a conventional PCR method, and determining the type of the salmonella serogroup in the sample. The determined type of the salmonella serogroup in the sample is concretely as follows: the gel electrophoresis detection is carried out, wherein if 350bp and 466bp bands exist, the result shows that the serogroup A exists; if 177bp bands exist, the result shows that the serogroup B exists; if 623bp bands exist, the result shows that the serogroup C1 exists; if 540bp bands exist, the result shows that the serogroup C2 exists; and if 466bp bands exist, the result shows that the serogroup D exists. The method of the invention can be used for quickly and accurately identifying the salmonella of the serogroups A, B, C1, C2 or D, and avoids the defects of complicated operation, time and labor consumption and high cost of the existing method.

The invention discloses a PCR (polymerase chain reaction) detection method for specificity of salmonella typhimurium. The PCR detection method includes steps: designing amplifier set sequences SEQ ID NO:2 and SEQ ID NO:3 according to a conservative and specific STM (scanning tunneling microscope) 4493 gene sequence SEQ ID:1 in a salmonella typhimurium complete genome sequence; extracting a DNA (deoxyribose nucleic acid) sample, and realizing amplification by a PCR method; and detecting an amplified product by means of gel electrophoresis and judging whether the sample contains the salmonella typhimurium or not. Particularly, if a single amplification band appears at 456bp in an electrophoresis result, the sample contains the salmonella typhimurium; and if the corresponding amplification band does not appear, the sample does not contain the salmonella typhimurium. When applied to detect the salmonella typhimurium, the PCR detection method has the advantages of short detection time, low cost, capability of detecting specificity of the detection result and simplicity in judging the result.

The invention discloses a specific primer, method and application for detecting Acinetobacter towneri. The specific primer is shown in SEQ ID NO.1-2. By extracting the genomic DNA of the sample to be tested, the above primer is used for PCR Amplify, detect by gel electrophoresis, and detect by taking pictures under the gel imaging system. If there is a 401bp DNA-specific band, it is determined that the sample contains Acinetobacter towneri. The detection method of the invention requires short time for detection of Acinetobacter towneri, strong specificity and high sensitivity. The invention avoids the disadvantages of cumbersome operation, long time consumption, low accuracy and low detection rate of the traditional identification method.

The invention relates to a primer for detection, specifically relates to a PCR (polymerase chain reaction) detection primer for detecting enterobacter cloacae and belongs to the technical field of biological detection. The enterobacter cloacae specific PCR detection primer comprises a forward primer and a reverse primer, wherein the forward primer F is 5'-CATGACACCGGTGTTTCCCCAGT-3'; and the reverse primer R is 5'-CGGTCGGTGAAGCCCAGAACCACTA-3'. A detection method for detecting the enterobacter cloacae, disclosed by the invention, has the advantages that the required detection time is short, the specificity is strong and the sensitivity is high. The PCR detection primer disclosed by the invention can avoid the shortcomings of trivial operation, long time, low accuracy, low detection rate and the like of a traditional identification method.