A kind of primer and method for detecting Enterobacter cloacae O20 type
A technology of Enterobacter cloacae and forward primer, which is applied in the field of primers for detecting Enterobacter cloacae O20 type, can solve the problems of inability to type Enterobacter cloacae, weak typing ability, unsatisfactory reproducibility, etc., so as to reduce the detection cost. , easy to determine, avoid the effect of cumbersome operation
Inactive Publication Date: 2020-02-28
TIANJIN CITY THIRD CENT HOSPITAL
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However, the above typing methods can only be used in combination. Due to the unsatisfactory reproducibility and weak typing ability of using one method alone, it cannot accurately type Enterobacter cloacae.
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[0018] The technical solutions of the present invention will be further described below in conjunction with specific embodiments. The implementation method that does not indicate specific condition in the embodiment, generally according to molecular cloning such as conventional conditions such as Sambrook, the condition described in the laboratory handbook (New York: Cold Spring Habor Laboratory Press, 1989), or according to the condition suggested by the manufacturer .
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The invention discloses primers and a method for detection of enterobacter cloacae O20 type, wherein the primers comprise one of two groups of nucleotide sequences, a forward primer of the first group of nucleotide sequences is 5'-AGAATCTTACACTGGCTTA-3', and the reverse primer is 5'-CTTAATACACCTGGCACA-3'; the forward primer of the second group of nucleotide sequences is 5'-TTTGGCTATGGCCGTATT-3', and the reverse primer is 5'-GTGCAGATGCCTGATGAA-3'. During the detection, DNA of a to-be-detected sample is extracted, and the PCR amplification is performed by using the first group of nucleotide sequences or the second group of nucleotide sequences as the primers, and the amplified product is subjected to electrophoresis detection. The method avoids the disadvantages of a traditional identification method, and has the advantages of short time, high specificity and high sensitivity for detecting the enterobacter cloacae O20 type.
Description
technical field [0001] The invention relates to the technical field of biological detection, in particular to a primer and a method for detecting Enterobacter cloacae O20 type. Background technique [0002] Enterobacter cloacae (Enterobacter cloacae), as a Gram-negative stubby bacillus, widely exists in nature and can be detected in soil, plants, insects, and human and animal feces. It is one of the species, and it is also an important opportunistic pathogen. In recent years, due to the widespread clinical use of extended-spectrum antibacterial drugs such as third- and fourth-generation cephalosporins and carbapenems, the number of drug-resistant strains of this bacteria is increasing, which makes the clinical infection caused by this bacteria Disease treatment tends to be complicated. The main reason for the emergence of drug-resistant strains is the failure to identify the pathogenic bacteria at the first time, and in the case of unknown pathogenic bacteria, drug abuse. ...
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IPC IPC(8): C12Q1/689C12Q1/686C12Q1/10C12Q1/04C12N15/11C12R1/01
CPCC12Q1/686C12Q1/689C12Q2565/125
Inventor 李雅玥王晓彤李彤
Owner TIANJIN CITY THIRD CENT HOSPITAL
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