Primer and method for detecting enterobacter cloacae O12
An Enterobacter cloacae and a technology to be detected, applied in the field of primers for detecting Enterobacter cloacae O12 type, can solve the problems of weak typing ability, inability to type Enterobacter cloacae, unsatisfactory reproducibility, etc., and achieves easy determination and practicality. Strong sex, avoid the effect of tedious operation
Inactive Publication Date: 2017-05-03
TIANJIN CITY THIRD CENT HOSPITAL
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Problems solved by technology
However, the above typing methods can only be used in combination. Due to the unsatisfactory reproducibility and weak typing ability of using one method alone, it cannot accurately type Enterobacter cloacae.
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[0015] The technical solutions of the present invention will be further described below in conjunction with specific embodiments. The implementation method that does not indicate specific condition in the embodiment, generally according to molecular cloning such as conventional conditions such as Sambrook, the condition described in the laboratory handbook (New York: Cold Spring Habor Laboratory Press, 1989), or according to the condition suggested by the manufacturer .
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The invention discloses a primer and method for detecting enterobacter cloacae O12. The primer is composed of a forward primer 5'-CGTTCGCAGAATACAGAG-3' and a reverse primer 5'-TCAAAGCAAACAGGGAGT-3'. The method includes: extracting DNA of a to-be-detected sample, utilizing the primer to conduct PCR amplification, and subjecting the amplification product to agarose gel electrophoresis detection. The detection method provided by the invention for detection of enterobacter cloacae O12 has the characteristics of short time, strong specificity and high sensitivity, and avoids the disadvantages of tedious operation, long time, low accuracy, low detection rate and the like of traditional identification methods.
Description
technical field [0001] The invention relates to the technical field of biological detection, in particular to a primer and a method for detecting Enterobacter cloacae O12 type. Background technique [0002] Enterobacter cloacae (Enterobacter cloacae), as a Gram-negative stubby bacillus, widely exists in nature and can be detected in soil, plants, insects, and human and animal feces. It is one of the species, and it is also an important opportunistic pathogen. In recent years, due to the widespread clinical use of extended-spectrum antibacterial drugs such as third- and fourth-generation cephalosporins and carbapenems, the number of drug-resistant strains of this bacteria is increasing, which makes the clinical infection caused by this bacteria Disease treatment tends to be complicated. The main reason for the emergence of drug-resistant strains is the failure to identify the pathogenic bacteria at the first time, and in the case of unknown pathogenic bacteria, drug abuse. ...
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IPC IPC(8): C12Q1/68C12Q1/04C12N15/11C12R1/01
CPCC12Q1/686C12Q1/689C12Q2565/125
Inventor 李雅玥王晓彤韩涛
Owner TIANJIN CITY THIRD CENT HOSPITAL