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Enterobacter cloacae specific PCR (polymerase chain reaction) detection primer

A technology for the detection of Enterobacter cloacae and primers, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., and can solve the problems related to the nucleic acid and primer related reports of PCR detection methods for Enterobacter cloacae that have not yet been invented. Achieve the effects of strong practicability, easy judgment, and avoid cumbersome operations

Active Publication Date: 2014-02-26
ZHEJIANG INST OF FRESH WATER FISHERIES
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Found through the document retrieval of prior art, have not yet invented the report related to PCR detection method, nucleic acid and primer of Enterobacter cloacae of the present invention

Method used

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  • Enterobacter cloacae specific PCR (polymerase chain reaction) detection primer
  • Enterobacter cloacae specific PCR (polymerase chain reaction) detection primer
  • Enterobacter cloacae specific PCR (polymerase chain reaction) detection primer

Examples

Experimental program
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Effect test

Embodiment 4

[0039] Example 4: Detection of suspected clinical strains

[0040] The 13 suspected strains isolated from the larvae of Macrobrachium rosenbergii were detected by using the specific PCR detection method for Enterobacter cloacae established in Example 1. See the test results image 3 , lane M in the figure is the DL2000 molecular weight standard, lane 1 is the positive control of Enterobacter cloacae, lane 2 is the negative control whose template is sterilized double distilled water, and lanes 3-15 are 13 suspected strains. As can be seen in the figure, 6 strains including Nos. 5, 9, 10, 11, 12, and 15 were positive.

[0041] It should be understood that those skilled in the art can make improvements or changes based on the above description, and all these improvements and changes should belong to the protection scope of the appended claims of the present invention.

[0042] Zhejiang Freshwater Fisheries Research Institute

[0043]

[0044] A specific PCR detection primer f...

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Abstract

The invention relates to a primer for detection, specifically relates to a PCR (polymerase chain reaction) detection primer for detecting enterobacter cloacae and belongs to the technical field of biological detection. The enterobacter cloacae specific PCR detection primer comprises a forward primer and a reverse primer, wherein the forward primer F is 5'-CATGACACCGGTGTTTCCCCAGT-3'; and the reverse primer R is 5'-CGGTCGGTGAAGCCCAGAACCACTA-3'. A detection method for detecting the enterobacter cloacae, disclosed by the invention, has the advantages that the required detection time is short, the specificity is strong and the sensitivity is high. The PCR detection primer disclosed by the invention can avoid the shortcomings of trivial operation, long time, low accuracy, low detection rate and the like of a traditional identification method.

Description

technical field [0001] The invention relates to a primer for detection, in particular to a primer for PCR detection of Enterobacter cloacae, belonging to the technical field of biological detection. Background technique [0002] Enterobacter cloacae is a Gram-negative bacterium that exists widely in nature and can be detected in human and animal feces, soil, plants, and insects. It is one of the normal intestinal bacteria of humans and animals. It is also an important opportunistic pathogen. In recent years, due to the widespread clinical use of extended-spectrum antibacterial drugs such as third-generation and fourth-generation cephalosporins and carbapenems, the bacteria have continuously developed drug-resistant mechanisms under their selection pressure, and drug-resistant strains Increasingly, it has become an important nosocomial infection pathogen, and the bacterial infectious diseases caused by it often involve multiple organs, including skin and soft tissue infectio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12Q1/10C12N15/11
CPCY02A50/30
Inventor 陈雪峰杨国梁王军毅高强张宇飞黄雪娜
Owner ZHEJIANG INST OF FRESH WATER FISHERIES
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