60 results about "Enterobacter cloacae subsp. dissolvens" patented technology

The invention discloses enterobacter cloacae having a phosphorus solubilizing function and used for degrading imazethapyr and relates to enterobacter cloacae. The enterobacter cloacae have the functions of degrading imazethapyr and degrading indissolvable phosphorus into soluble phosphorus, thereby laying a foundation for solving the problem of soil pollution caused by residues of a herbicide imazethapyr by means of micro-biological degradation. The enterobacter cloacae T02 are preserved in CGMCC with the preserving address: #3, Yard 1, Beichen West Road, Chaoyang District, Beijing on Jule 9, 2014 and have a preservation number: CGMCC No. 9287. The enterobacter cloacae T02 not only have the function of degrading imazethapyr, but also have the function of degrading indissolvable phosphorus into soluble phosphorus. The enterobacter cloacae are used for degrading residual herbicide imazethapyr in soil.

The invention relates to a primer for detection, specifically relates to a PCR (polymerase chain reaction) detection primer for detecting enterobacter cloacae and belongs to the technical field of biological detection. The enterobacter cloacae specific PCR detection primer comprises a forward primer and a reverse primer, wherein the forward primer F is 5'-CATGACACCGGTGTTTCCCCAGT-3'; and the reverse primer R is 5'-CGGTCGGTGAAGCCCAGAACCACTA-3'. A detection method for detecting the enterobacter cloacae, disclosed by the invention, has the advantages that the required detection time is short, the specificity is strong and the sensitivity is high. The PCR detection primer disclosed by the invention can avoid the shortcomings of trivial operation, long time, low accuracy, low detection rate and the like of a traditional identification method.

The invention discloses a bagasse immobilized bacterium and application thereof to sulfamethoxazole pollution remediation of farmland soil. According to the bagasse immobilized bacterium, Enterobacter cloacae is immobilized by adopting bagasse, and when the Enterobacter cloacae is inoculated to a bagasse vector, a bacterial suspension is prepared by culturing in advance, and then the Enterobacter cloacae is immobilized onto the bagasse vector treated by an inorganic salt culture solution to establish an efficient and stable agricultural waste immobilized bacterium system. The bagasse immobilized bacterium is low in cost, and when the soil is remediated, simplicity and environment-friendliness are achieved, and secondary pollution is avoided; in addition, the bioavailability of sulfonamides can be improved, and the microbiological degradation efficiency of the sulfonamides is improved.

The invention discloses saline-alkaline tolerant enterobacter cloacae and a production method and application of a viable bacterium preparation of the saline-alkaline tolerant enterobacter cloacae. The saline-alkaline tolerant enterobacter cloacae ZTS-10 is separated from rhizosphere soil of chili peppers in saline-alkaline land, the preservation number of the bacterial strain in the China GeneralMicrobiological Culture Collection Center (CGMCC) is CGMCC No.20181, and the preservation date is 3 July, 2020. The bacterial strain is high in saline-alkaline tolerance and high in field planting rate in the saline-alkaline soil, has the capacity of producing IAA, degrading phosphorus and degrading potassium, can promote growth of the chili peppers, has good antagonism effects on pathogenic bacteria of chili pepper epidemic diseases, can be used for producing microorganism fertilizers especially microorganism fertilizers special for the chili peppers in the saline-alkaline land, can reduce the generation of chili pepper epidemic diseases, and can increase yield and quality.

The invention discloses enterobacter cloacae with the aerobic denitrification function and an application of the enterobacter cloacae in wastewater treatment. The enterobacter cloacae are enterobacter cloacae HNR and are collected to the China General Microbiological Culture Collection Center (CMGCC) on January 13th, 2015, the collecting number is CGMCC No.10348. According to the enterobacter cloacae, under the aerobic condition, NO3<->-N can be converted into a gaseous product through the aerobic denitrification effect with organic carbon as the unique carbon source and the NO3<->-N (nitrate nitrogen) as the unique nitrogen source, and synchronous removing of nitrogen elements and the organic carbon is achieved. According to the enterobacter cloacae, the removing rate for the NO3<->-N is as high as 90% to 98 %, the removing rate for the total nitrogen can be 85% to 95 %, and the removing rate for the total organic carbon can be higher than 85%. The enterobacter cloacae can be used for effectively removing the NO3<->-N in the organic wastewater, and have the effects that the whole process is aerobic, and the nitrogen removal efficiency is high.

The invention relates to enterobacter cloacae which are found in soil and have the collection number of CGMCC No.2487 or resting cell with equivalent function, variant and mutant as well as enzyme produced thereby. The invention also provides a preparation method of taxane, namely comprising the following steps: the enterobacter cloacae are used for hydrolyzing 7-xylose group in 7-xylose taxane molecule in wheat bran culture medium, and 13-side chain in taxane molecule with 13-side chain can be hydrolyzed in peptone beef extract culture medium; and the produced taxane can be used for preparing antitumor drugs, such as intermediates of taxane drugs, docetaxel drugs or taxane drugs.

The invention discloses an alga-lysing microbial agent and application thereof. The microbial agent contains enterobacter and/or an enterobacter extract, and the enterobacter is selected from enterobacter hormaechei F2 or enterobacter cloacae. The microbial algicidal inoculant contains the enterobacter hormaechei F2 and/or the enterobacter hormaechei F2 extract, and has an obvious dissolution effect on microcystis aeruginosa in a water body, the algicidal rate reaches 95%, the algicidal rate on filamentous algae reaches 97%, the removal rate of algal cells in an actual eutrophic water body can reach 87.32%, and the removal rate of the microcystis aeruginosa in the actual eutrophic water body can reach 87.32%. The alga-lysing microbial agent has great application in water eutrophication treatment.

The invention discloses bacterium with an induced resistance activity and a cultural method and application thereof, wherein the bacterium is enterobacter cloacae and the preservation number of the enterobacter cloacae is CGMCC No. 10801. The bacterium with an induced resistance activity or the metabolite thereof can be used for preparation of a soybean seed treating agent and biological prevention and control of plant parasitic nematodes. The enterobacter cloacae (E.cloacae) Sneb572 or the metabolite thereof has an activity of inducing soybeans, can be used for soybean seed treatment, therefore not only is the growth of soybean plants and roots promoted, but also a great inhibiting effect on formation of heterodera glycines cysts is achieved, and the problem of prevention and control of heterodera glycines is solved.

The invention discloses enterobacter cloacae with capability of degrading a benzene series and application of the enterobacter cloacae in environmental restoration. According to the enterobacter cloacae and the application thereof, the enterobacter cloacae with good capability of degrading the benzene series is screened and separated from landfill leachate of a certain refuse landfill in Guangzhou, Guangdong; and the enterobacter cloacae GDUTAN7 is preserved in the China Center for Type Culture Collection (CCTCC) on January 18, 2021, the address is Wuhan University, Wuhan, China, and the preservation number is CCTCC NO: M 2021091. The enterobacter cloacae GDUTAN7 disclosed by the invention has a relatively good degradation effect on the benzene series in environments and can be applied to the degradation of the benzene series in the environments, and thus, the aim of restoring the environments is achieved.

The invention relates to enterobacter cloacae for degrading petroleum pollution and application thereof. The enterobacter cloacae 2# provided by the invention has a preservation number of CGMCC No. 18937, and the strain (enterobacter cloacae 2#) has an effect of degrading petroleum. The strain can be applied to the treatment of petroleum pollution, and the strain can be removed through natural degradation after the petroleum is degraded by the strain, so that the strain can be used for the treatment of the petroleum-polluted environment without causing secondary pollution to the environment.

The invention discloses a preparation method and application of Leersia hexandra swartz endophytic bacteria with a hexavalent chromium reducing function. The strain has the classification naming of Enterobacter cloacae, and is collected in CGMCC (China General Microbiological Culture Collection Center) with the address of NO.3, NO.1 Yard, Beichen West Road, Chaoyang District, Beijing, 100101. Thecollection number is CGMCC NO.14265. The Leersia hexandra swartz endophytic bacteria are separated and screened from the root tissues of the chromium super accumulation plants of Leersia hexandra swartz, are identified as Enterobacter cloacae, and are named as G04. The strain can reduce hexavalent chromium into trivalent chromium; the chromium reducing conditions are cleared; the strain resource and the theoretical basis are provided for hexavalent chromium pollution microbial remediation.

The invention discloses an escherichia coli engineering bacterium for efficiently producing 2,3,5,6-tetramethylpyrazine. The engineering bacterium is an escherichia coli carrying an acetoin syntheticoperator alsRSD from Enterobacter cloacae and an NADH oxidase gene nox from Lactobacillus brevis. Using the genetic engineering bacterium provided by the invention to transform glucose to produce 2,3,5,6-tetramethylpyrazine, the highest yield is up to 16.40g/L, the purity of a product is more than or equal to 98%, and the engineering bacterium has the advantages of mild reaction conditions, few by-products, and easy separation and purification of the product, and has a very good application value.

The invention discloses a method for enhancing colonization of enterobacter cloacae Y16 in a plant root system. Research finds that when bacterial liquid of enterobacter cloacae Y16 and low-molecular organic acid are jointly applied to the rhizosphere soil of a heavy metal hyperaccumulation plant, the formation of a biological membrane of the enterobacter cloacae in the plant root system can be remarkably promoted, the colonization ability of the enterobacter cloacae is enhanced, the growth of the plant is promoted, and the efficiency of microorganism-plant combined remediation of the farmland soil polluted by medium-low-concentration cadmium is improved. The low-molecular organic acid is at least one of malic acid, citric acid or succinic acid which has a directional recruitment effect on the enterobacter cloacae Y16. According to the method, the remediation efficiency of the heavy metal hyperaccumulation plant on the medium-low heavy metal polluted farmland soil can be remarkably improved.

The invention relates to the field of uranium mine radioactive pollution treatment, in particular to enterobacter cloacae and application thereof. The collection number of the enterobacter cloacae is CGMCC (China General Microbiological Culture Collection Center) NO: The enterobacter cloacae is applied to uranium element reduction and uranium ore slag pollution treatment. The invention provides a preparation method of active bacterial liquid, which comprises the following steps: inoculating enterobacter cloacae into a liquid culture medium, standing and culturing to obtain a basic active bacterial agent; and inoculating the basic active bacterial agent into a fermentation medium according to an inoculation amount of 2-5%, and carrying out shake culture, so that the number of cells in each milliliter of the fermentation medium is not less than 3 * 10 < 9 >-5 * 10 < 9 >, thereby obtaining the active bacterial liquid. The strain has a reduction effect on uranium in tailings, uranium is converted into a reduction state which is not easy to dissolve from an oxidation state which is easy to dissolve, and the uranium is fixed in situ, so that the risk of migration to the external environment is reduced, and uranium pollution is inhibited from the source.

The invention discloses application of luteolin in inhibiting the growth of multi-drug resistant enterobacter cloacae. Luteolin has a good in-vitro killing effect on multi-drug resistant enterobactercloacae with resistance to cefazolin, cefotaxime, augmentin, meropenem, ofloxacin, levofloxacin, ciprofloxacin, cefoxitin, minocycline, imipenem, piperacillin, azithromycin, furantoin, sulfamethoxazole and nalidixic acid, can inhibit the growth of the multi-drug resistant enterobacter cloacae, and has a minimum bactericidal concentration of 0.5mg/mL and a minimum inhibitory concentration of 0.3mg/mL. The invention provides the inhibition effect of luteolin on the multi-drug resistant enterobacter cloacae, and has wide application value in the fields of medicine and the like.

The invention relates to a primer composition for detecting carbapenemase-resistant enterobacter cloacae OXA48 family genes through LAMP, a corresponding kit and a detection method, common gene segments of the OXA48 family are selected, the common gene segments comprise blaOXA-48, blaOXA-181, blaOXA-232, blaOXA-204, blaOXA-162 and blaOXA-244, primers needed in a loop-mediated isothermal amplification system are designed, and the primers are used for detecting carbapenemase-resistant enterobacter cloacae OXA48 family genes through LAMP. A reaction system, reaction conditions, coloring agent concentration and the like are repeatedly optimized, and the improved OXA48 family LAMP detection kit is established. The LAMP detection kit not only can be used for detecting OXA48 family genes of enterobacter cloacae, but also can be used for detecting other bacteria, and the bacteria containing the drug-resistant genes can be detected. The LAMP detection kit is simple and convenient to operate and low in operation risk.