Colloidal gold test strip and detection method for silkworm nuclear polyhedrosis virus immunity

Abstract

The invention relates to an immune colloidal gold test strip and a detection method for karyotype polyhedrosis viruses of bombyx mori, particularly relates to an immune colloidal gold diagnosis test strip for detecting the karyotype polyhedrosis viruses of the bombyx mori by adopting an immunochromatography technology, and a preparation method of the immune colloidal gold diagnosis test strip, and belongs to the technical field of virus epidemic disease diagnosis. The immune colloidal gold test strip comprises a colloidal gold pad marked with an antigen BmNPV-Lef4 antibody and a coated nitrocellulose membrane, wherein an upper detection line of the nitrocellulose membrane is coated by rabbit-anti BmNPV-Lef4; and a quality control line at the lower part of the nitrocellulose membrane is coated with goat-anti-mouse IgG. The immune colloidal gold test strip is used for detecting the karyotype polyhedrosis viruses of the bombyx mori; compared with a traditional colloidal gold detection method, a double-antibody sandwich method is adopted when the colloidal gold test strip is prepared and a concentration proportion of two virus antibodies is regulated, so that the specificity, the sensitivity and the stability of a detection result can be effectively guaranteed; the detection sensitivity is high and the detection method is simple and convenient and is applied to the detection of the karyotype polyhedrosis viruses of the bombyx mori for the first time.

Description

technical field [0001] The present invention relates to an immune colloidal gold detection test strip for silkworm nuclear polyhedrosis virus and a detection method thereof, in particular to an immune colloidal gold diagnostic test strip for detecting silkworm nuclear polyhedrosis virus by an immune chromatography technique and a preparation method thereof, The invention belongs to the technical field of virus epidemic diagnosis. Background technique [0002] The silkworm is an important economic insect, and the silk it produces is the main source of silk raw materials, and plays an important role in human economic life and history and culture. However, the high incidence of silkworm disease directly affects the yield and quality of silkworm cocoons, restricting the healthy and sustainable development of sericulture. Silkworm diseases are divided into infectious silkworm diseases and non-infectious silkworm diseases, with infectious silkworm diseases being the most importan...

AI Technical Summary

Problems solved by technology

So far, this technology has not been reported in the detection of silkworm nuclear polyhedrosis virus (BmNPV), and because the hemolymph and tissue of the silkworm are convenient to collect, especially the hemolymph is not easy to coagulate, the hemolymph of the silkworm can be directly collected for colloid In the detection of gold, it can greatly reduce the operation steps and use costs, and is more convenient for silkworm farmers and front-line technicians to master and use. Therefore, the diagnostic colloidal gold test strip has a good application prospect

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