PRV, PCV-2, PPV multiple PCR (Polymerase Chain Reaction) detection kit

A PCV-2 and detection kit technology, applied in the field of kits, can solve the problems of time-consuming, labor-intensive, low sensitivity, complicated operation, etc.

Inactive Publication Date: 2012-09-19
GUIZHOU INST OF ANIMAL HUSBANDRY & VETERINARY
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  • Abstract
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Problems solved by technology

However, it is difficult for conventional diagnostic methods to distinguish diseases with similar symptoms at the same time, and conventional et

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  • PRV, PCV-2, PPV multiple PCR (Polymerase Chain Reaction) detection kit
  • PRV, PCV-2, PPV multiple PCR (Polymerase Chain Reaction) detection kit
  • PRV, PCV-2, PPV multiple PCR (Polymerase Chain Reaction) detection kit

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Embodiment Construction

[0063] Embodiments of the present invention: PRV, PCV-2, PPV multiplex PCR detection kit, it comprises 40 μ L equal volumes of mixed PRV primers, PCV-2 primers and PPV primers, the concentration of PRV primers, PCV-2 primers and PPV primers is equal to The target gene of the PRV primer is gE, the sequence number of the upstream primer of the PRV primer is PRV-1:5'-CCCACGCACGAGGACTAC-3', the sequence number of the downstream primer of the PRV primer is PRV-2:5'-GGGCGGGACATCAACAGG- 3', the fragment size is 288bp; the target gene of the PCV-2 primer is ORF2, and the sequence number of the upstream primer of the PCV-2 primer is PCV-1: 5'-GGATTGTATGGCGGGAGG-3', the sequence number of the downstream primer of the PCV-2 primer For PCV-2: 5'-AGGAGGCGTTACCGAAGGAG-3', the fragment size is 419bp; the target gene of the PPV primer is VP2, and the sequence number of the upstream primer of the PPV primer is PPV-1: 5'-GGGAGGGCTTGGTTAGAATC-3', of the PPV primer The sequence number of the down...

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Abstract

The invention discloses a PRV, PCV-2, PPV multiple PCR (Polymerase Chain Reaction) detection kit which comprises 40 mu L of isopyknic mixed PRV primer, PCV-2 primer and PPV primer, wherein the concentration of the PRV primer, the PCV-2 primer and the PPV primer is 10muM, buffer solution is 600 mu L, negative control is 20 mu L, PCR enzyme is 250 mu L, ultrapure water is 170 mu L, Market DL 2000 is 50 mu L, and positive control is 20 mu L. The invention aims to a condition that the PRV,the PCV-2 and the PPV exist in a same reaction system, designs three types of primers, and adopts the kit made from the three types of primers. By utilizing, the PRV,the PCV-2 and the PPV can be simultaneously detected, the specificity and sensitivity are high, the detection time is short, the detection cost is low, and a scientific basis is provided for prevention and control of pig infectious diseases.

Description

technical field [0001] The invention relates to a reagent kit, in particular to a PRV, PCV-2 and PPV multiplex PCR detection reagent kit. Background technique [0002] With the continuous development of large-scale pig farming, the current reproductive obstacles caused by porcine pseudorabies virus (PRV), porcine circovirus (PCV-2) and porcine parvovirus (PPV) are relatively serious, and mixed infections often occur. The pig industry has caused huge economic losses. The mixed infection of PRV, PCV-2 and PPV is difficult to make a differential diagnosis based on clinical symptoms alone, and the large-scale application of PRV gene deletion vaccines makes it necessary to distinguish between wild virus infection and vaccination. At present, the main detection methods include virus isolation and identification, agar diffusion test, trace serum neutralization test, ELISA, and PCR methods. For PRV gene deletion vaccines, gE-ELISA is used to distinguish wild virus infection from va...

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/68C12R1/93
Inventor 余波谭诗文冉懋韬徐景峨王璇李干洲艾玉萍
Owner GUIZHOU INST OF ANIMAL HUSBANDRY & VETERINARY
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