Primers and method for detection of enterobacter cloacae O21 type
A technology to be detected for Enterobacter cloacae, which is applied in the field of primers for detecting Enterobacter cloacae O21 type, can solve the problems of weak typing ability, failure to type Enterobacter cloacae, unsatisfactory reproducibility, etc., and achieve easy determination and practicality Strong, to avoid the effect of cumbersome operation
Inactive Publication Date: 2017-05-03
TIANJIN CITY THIRD CENT HOSPITAL
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However, the above typing methods can only be used in combination. Due to the unsatisfactory reproducibility and weak typing ability of using one method alone, it cannot accurately type Enterobacter cloacae.
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[0015] The technical solutions of the present invention will be further described below in conjunction with specific embodiments. The implementation method that does not indicate specific condition in the embodiment, generally according to molecular cloning such as conventional conditions such as Sambrook, the condition described in the laboratory handbook (New York: Cold Spring Habor Laboratory Press, 1989), or according to the condition suggested by the manufacturer .
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The invention discloses primers and a method for detection of enterobacter cloacae O21 type, wherein the primers comprise a forward primer 5'-TGTCTTGACCGCCTAT-3' and a reverse primer of 5'-AAACCGAATGATAAGC-3'. During the detection, DNA of a sample is extracted, and the PCR amplification is performed by using the primers, and the amplified product is detected by 1% agarose gel electrophoresis. The detection method has the advantages of short required time, high specificity and high sensitivity for detecting the enterobacter cloacae O21 type, and the disadvantages of complicated operation, long time consuming, low accuracy, low detection rate and the like of a traditional identification method are avoided.
Description
technical field [0001] The invention relates to the technical field of biological detection, in particular to a primer and a method for detecting Enterobacter cloacae O21 type. Background technique [0002] Enterobacter cloacae (Enterobacter cloacae), as a Gram-negative stubby bacillus, widely exists in nature and can be detected in soil, plants, insects, and human and animal feces. It is one of the species, and it is also an important opportunistic pathogen. In recent years, due to the widespread clinical use of extended-spectrum antibacterial drugs such as third- and fourth-generation cephalosporins and carbapenems, the number of drug-resistant strains of this bacteria is increasing, which makes the clinical infection caused by this bacteria Disease treatment tends to be complicated. The main reason for the emergence of drug-resistant strains is the failure to identify the pathogenic bacteria at the first time, and in the case of unknown pathogenic bacteria, drug abuse. ...
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IPC IPC(8): C12Q1/68C12Q1/04C12N15/11C12R1/01
CPCC12Q1/686C12Q1/689C12Q2565/125
Inventor 李雅玥王晓彤
Owner TIANJIN CITY THIRD CENT HOSPITAL
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