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Enterobacter cloacae having phosphorus solubilizing function and used for degrading imazethapyr

A technology of Enterobacter cloacae and Imazethapyr, applied in the field of Enterobacter cloacae, can solve problems such as pollution

Active Publication Date: 2014-12-17
INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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  • Abstract
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  • Application Information

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Problems solved by technology

[0004] The invention provides an Enterobacter cloacae bacterial strain, which has the functions of degrading imazethapyr and degrading insoluble phosphorus into soluble phosphorus, and solves the residual soil pollution of the herbicide imazethapyr by microbial degradation. questions lay the groundwork

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  • Enterobacter cloacae having phosphorus solubilizing function and used for degrading imazethapyr

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specific Embodiment approach 1

[0015] Specific Embodiment 1: The Enterobacter cloacae used for the degradation of imazethapyr with the function of dissolving phosphorus in this embodiment is Enterobacter cloacae (Enterobactercloacae) T02. No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing. The preservation date is June 9, 2014, and the preservation number is CGMCCNo.9287.

[0016] In this embodiment, the Enterobacter cloacae T02 is a Gram-negative bacterium with a short rod shape and no spores. Enterobacter cloacae T02 grows on the beef extract peptone medium, the colony is moist, flat, with neat edges, 0.2-0.5 cm in diameter, slightly yellow. Enterobacter cloacae T02 is a mesophile with an optimum growth temperature of 30°C and an optimum pH of 7.0.

[0017] Physiological and biochemical identification of Enterobacter cloacae T02 was carried out with reference to the eighth edition of "Berger's Bacteria Identification Manual" and "Common Bacteria System Identification Manual": In this e...

specific Embodiment approach 2

[0018] Specific embodiment 2: In this embodiment, Enterobacter cloacae (Enterobacter cloacae) T02 is isolated from soybean field soil in Nehe City, Heilongjiang Province. The separation method is carried out as follows:

[0019] 1. Preliminary screening: After the soil sample is air-dried, crushed, and sieved, 5.0g of the soil sample is weighed and added to the basic salt medium with imazethapyr as the only carbon and energy source. After 5-7 days of liquid Shaker culture, culture temperature 30 ℃, rotation speed 160r / min;

[0020] Basal salt medium: composed of 0.1gK 2 HPO 4 , 0.04gCaCO 3 , 0.2gMgSO 4 ·7H 2 O, 0.1gNaCl, 0.001gFeSO 4 ·7H 2 O, 0.1g (NH 4 ) 2 SO 4 and 1000mLH 2 O composition, pH 7.0-7.2, sterilized at 121°C for 20 minutes. Before use, add 3mL of 10% imazethapyr to 100mL of basic salt medium, that is, the net content of imazethapyr in every 100ml of basic salt medium is 0.3g;

[0021] 2. Re-screening: 10 mL of the primary screening culture was subjec...

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Abstract

The invention discloses enterobacter cloacae having a phosphorus solubilizing function and used for degrading imazethapyr and relates to enterobacter cloacae. The enterobacter cloacae have the functions of degrading imazethapyr and degrading indissolvable phosphorus into soluble phosphorus, thereby laying a foundation for solving the problem of soil pollution caused by residues of a herbicide imazethapyr by means of micro-biological degradation. The enterobacter cloacae T02 are preserved in CGMCC with the preserving address: #3, Yard 1, Beichen West Road, Chaoyang District, Beijing on Jule 9, 2014 and have a preservation number: CGMCC No. 9287. The enterobacter cloacae T02 not only have the function of degrading imazethapyr, but also have the function of degrading indissolvable phosphorus into soluble phosphorus. The enterobacter cloacae are used for degrading residual herbicide imazethapyr in soil.

Description

technical field [0001] The invention relates to a strain of Enterobacter cloacae. Background technique [0002] Chemical weeding is currently the most important method to deal with weeds in farmland. It plays a very important role in high agricultural yield and harvest, improving the degree of agricultural intensification, improving the quality of life of farmers, and reducing labor intensity. From the perspective of Heilongjiang Province, due to the relatively contiguous distribution of farmland and cultivated land, the land is fertile and flat. It is especially suitable for intensive and mechanized production, so the usage of chemical herbicides is very large. However, with the extensive use of long-residual herbicides for many years, herbicide residues accumulate in the farmland soil, causing crop phytotoxicity, resulting in crop yield reduction, and making it difficult for crops to rotate crops, which has become a difficult problem in many areas. At the same time, the ...

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Application Information

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IPC IPC(8): B09C1/10C12N1/20C12R1/01
Inventor 牛彦波吴皓琼曹亚彬殷博安琦郭立姝甄涛
Owner INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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