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Enterobacter cloacae, selenium-rich polysaccharide thereof and use of selenium-rich polysaccharide

A technology of Enterobacter cloacae and selenium enrichment, which is applied in the field of microbiology and bioengineering, can solve the problems of low bioavailability, and achieve the effects of enhancing function, improving lymphocyte proliferation ability, cytokine level, and oxidative damage protection

Inactive Publication Date: 2009-07-08
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The toxicity of inorganic selenium compounds is much higher than that of organic selenium compounds, and their bioavailability is low

Method used

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  • Enterobacter cloacae, selenium-rich polysaccharide thereof and use of selenium-rich polysaccharide
  • Enterobacter cloacae, selenium-rich polysaccharide thereof and use of selenium-rich polysaccharide
  • Enterobacter cloacae, selenium-rich polysaccharide thereof and use of selenium-rich polysaccharide

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, the preparation method of Enterobacter cloacae Z0206 selenium-enriched polysaccharide

[0030] (1) Selenium tolerance acclimation of Enterobacter cloacae Z0206: PDA enriched medium formula is as follows: potato 20%, peptone 0.3%, yeast extract 0.3%, glucose 2%, agar powder 1.6%. Enterobacter cloacae Z0206 strains were picked and spread on PDA enriched medium plates with selenium content of 10, 20, 30, 40, 50 μg / mL successively to carry out selenium concentration gradient acclimatization. Each gradient was incubated at 32°C for 36h. Finally, the well-grown colonies were picked from the PDA enriched medium plate with a selenium content of 50 μg / mL, streaked and separated on the plate, and preserved on the slant after purification.

[0031] (2) Study on selenium enrichment of Enterobacter cloacae Z0206:

[0032] Pick out the domesticated selenium-tolerant strains from the slope, inoculate them on the PDA enriched medium with selenium content of 10, 15, 20, 25...

Embodiment 2

[0047] Example 2. Detection of polysaccharide content in Enterobacter cloacae Z0206 selenium-enriched polysaccharide by phenol-sulfuric acid method

[0048] The method is as follows: Accurately weigh 20 mg of standard glucose in a 500ml volumetric flask, add water to the mark, absorb 0.2ml, 0.4ml, 0.6ml, 0.8ml, 1.0ml, 1.2ml, 1.4ml, 1.6ml, 1.8ml and add water to make up to 2.0ml, then add 1.0ml of 6.0% phenol and 5.0ml of concentrated sulfuric acid, let it stand for 10min, shake well, bathe in water for 30min, measure the optical density at 490nm after 20min, use 2.0ml of water as a blank, and the abscissa x is the polysaccharide content , and the ordinate y is the optical density value, and a standard curve is drawn. Draw 1.0ml of the sample solution (equivalent to about 40 μg of selenium-enriched polysaccharide), operate according to the above steps, measure the optical density, and calculate the polysaccharide content with the standard curve.

Embodiment 3

[0049] Example 3. Determination of the protein content in the selenium-enriched polysaccharide of Enterobacter cloacae Z0206 by the Kjeldahl method.

[0050] The Kjeldahl method (GB / T 5009.5-1985) stipulated in the national standard was used to determine the protein content in the selenium-enriched polysaccharide of Enterobacter cloacae Z0206.

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Abstract

The invention discloses an Enterobacter cloacae Z0206, which has been stored in 'China microorganism strain preservation management committee common micro organism center' at December 3rd, 2007 and whose storage number is CGMCC NO 2279; also discloses is an Enterobacter cloacae Selenium-rich polysaccharide preparation method and applications of Selenium-rich polysaccharide. The invention has advantages that: the invention has simple production process of extracting the Selenium-rich polysaccharide, high product yield and higher purity, higher Selenium-rich levels; the selenium-enriched polysaccharide prepared by the method can significantly improve the lymphopoiesis capability as well as cytokine level of mice with low immune capacity, and has significant role in the regulation of the immune function of mice with low immune capacity; the selenium-enriched polysaccharide prepared by the method can significantly improve the activity of anti-oxidase, to enhance the function of the antioxidant defense system, has a clear protective effect to the oxidative damage of RAW264.7 cells induced by H2O2, which is an effective antioxidant.

Description

technical field [0001] The invention relates to the technical fields of microbiology and bioengineering, and is specially designed to prepare and apply Enterobacter cloacae Z0206 bacteria with anti-oxidation and immune regulation functions through the selenium-resistant domestication of bacteria, selenium-enrichment research, and selenium-enriched polysaccharide extraction and purification process research. Method for activating selenium-enriched polysaccharides. Background technique [0002] As an important trace element, selenium has been paid more and more attention by the scientific community. Studies have shown that selenium has the functions of improving the body's immunity, anti-oxidation, detoxification, inhibiting tumors, and preventing cardiovascular diseases. The toxicity of inorganic selenium compounds is much higher than that of organic selenium compounds, and their bioavailability is low. Only by converting inorganic selenium into organic selenium can it have...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P19/04A23L1/29A23L1/30A61K31/715A61P35/00A61P31/12C12R1/01
Inventor 汪以真徐春兰靳明亮姚国佳
Owner ZHEJIANG UNIV
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